Lyons1: Plasma lipids and susceptibility to gallstones in F2 progeny of DBA/2J x CAST/EiJ (2003)

References   (chronological)

1.   Lyons MA, Wittenburg H, Li R, Walsh KA, Churchill GA, Carey MC, Paigen B. Quantitative trait loci that determine lipoprotein cholesterol levels in DBA/2J and CAST/Ei inbred mice. J Lipid Res. 2003 May;44(5):953-67. Epub 2003 Feb 16.   PubMed 12588951  

2.   Lyons MA, Wittenburg H, Li R, Walsh KA, Leonard MR, Korstanje R, Churchill GA, Carey MC, Paigen B. Lith6: a new QTL for cholesterol gallstones from an intercross of CAST/Ei and DBA/2J inbred mouse strains. J Lipid Res. 2003 Sep;44(9):1763-71. Epub 2003 Jun 16.   PubMed 12810825  

3.   Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16.   PubMed 15654110     FullText


     
Lyons1 downloads
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Use the QTL Data Downloads button near the bottom of this page.
Participants M A Lyons
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Li R, Wittenburg H, Paigen B
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsFunding provided by NIH DK51568, DK36588, DK52911, DK34854, CA34196, GM070683 and HL55001; and the American Physiological Society, American Liver Foundation, and Deutsche Forschungsgemeinschaft
Project type QTL Archive data set
MPD identifiersLyons1     MPD:130
Data changelog No updates/corrections.       Initial release date: 01/2008.
An intercross of CAST/EiJ and DBA/2J was generated to identify cholesterol gallstone susceptibility loci. F2 progeny (males only) were administered a high-fat (15%) diet for 10 wks and examined for gallstone formation and related phenotypes. Gallstones were characterized qualitatively and quantitatively, and lipid profiles determined for plasma samples. Mice were fasted prior to sample collection. For detailed protocols see associated publications (below).


QTL data set parameters:
Progenitor strains CAST/EiJ     DBA/2J    
Cross direction notation(CASTxDBA2) F1 x (CASTxDBA2) F1; (DBA2xCAST) F1 x (DBA2xCAST) F1
Cross typeF2 intercross
Population size 0 ♀     278 ♂
Age16-18wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
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Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Lyons1 13001 organ weights high fat diet  10wks liver weight   [g]  liver_wt QTL population m 16-18wks N=278
Lyons1 13002 histopathology high fat diet  10wks gallstones, presence (score 0=absent, 1=present)   [score]  gallstone_presence QTL population m 16-18wks N=278
Lyons1 13003 histopathology high fat diet  10wks gallstones, screening (score 0=absent, 1=present, 2=solid gallstones)   [score]  gallstone_score QTL population m 16-18wks N=278
Lyons1 13004 histopathology high fat diet  10wks number of gallstones   [n]  gallstone_count QTL population m 16-18wks N=278
Lyons1 13005 histopathology high fat diet  10wks weight of gallstones   [mg]  gallstone_weight QTL population m 16-18wks N=278
Lyons1 13006 histopathology high fat diet  10wks sandy gallstones (score 0=absence, 1-4=severity)   [score]  gallstone_sandy QTL population m 16-18wks N=278
Lyons1 13007 histopathology high fat diet  10wks solid gallstones (score 0=absence, 1-4=severity)   [score]  gallstone_solid QTL population m 16-18wks N=278
Lyons1 13008 histopathology high fat diet  10wks cholesterol monohydrate crystals, aggregates (score 0=absence, 1-4=severity)   [score]  ChMC_agg QTL population m 16-18wks N=278
Lyons1 13009 histopathology high fat diet  10wks cholesterol monohydrate crystals (score 0=absence, 1-4=severity)   [score]  ChMC_ind QTL population m 16-18wks N=278
Lyons1 13010 lipid profile high fat diet  10wks total cholesterol (plasma)   [mg/dL]  chol QTL population m 16-18wks N=278
Lyons1 13011 lipid profile high fat diet  10wks HDL (plasma), log transformed   [log(mg/dL)]  HDL_log QTL population m 16-18wks N=278
Lyons1 13012 lipid profile high fat diet  10wks nonHDL (plasma)   [mg/dL]  nonHDL QTL population m 16-18wks N=278
To see more rows use the paging control at top.

Associated genomic features
MGI-curated genes / genomic features that have been associated with this data set:
Chol6   Hdl1   Hdlq10   Hdlq11   Hdlq12   Lith1   Lith10   Lith11   Lith6  


Project / data set:   Lyons1     a.k.a. Lyons_2003
Excel file:   Data_Description_CASTxDBA_Lyons2003.xlsx
CSV file:   Lyons2003_CASTxDBA_B37_Data.csv
Readme / text file:   readme_Lyons2003.txt
QC plots, figures, etc.:   QCReport_Lyons2003.pdf