See JAX Pipeline on the IMPC website for order of tasks for all mice tested
Project protocol: Auditory brainstem response
Introduction
Auditory brainstem response test determines hearing sensitivity and other physiological parameters using evoked potential recordings in anesthetized mice.
Study design
Paradigm: Baseline study
Panel: Collaborative Cross
Number of strains: 18
Sex: Both
Age: 15-17 wks
Sample size: Three mice per sex
Number of cohorts: 1
Workflow
Step |
Description |
Equipment |
Data collected |
1 |
Mice are anesthetized and weighed |
- |
- |
2 |
Each mouse is prepped for ABR |
Auditory brainstem response system |
- |
3 |
ABRs are recorded |
Auditory brainstem response system |
Auditory brainstem response at 6, 12, 18, 24, 30 kHz |
Equipment, software and supplies
- Auditory brainstem response system [See Experimental and technical parameters]
- Audio signal generators, amplifiers and loudspeakers
- Calibration equipment (microphone, etc)
- EEG Needle electrodes
- Biological amplifier and headstage
- Sound attenuating chamber
- Heating blanket
- Data acquisition hardware
- Software to control signal presentation and data acquisition
- Data Analysis software/database application
Procedure
- Mice are anesthetized i.p. with ketamine-xylazine.
- Each mouse is placed on a heating blanket in a sound chamber and sub dermal needle electrodes are inserted (active electrode on vertex, reference electrode overlying left bulla, ground electrode overlying right bulla).
- Each mouse is placed unrestrained in a prone position, nose forward, at the calibrated distance from the leading edge of the speaker to the mouse's interaural axis, on a thermostatically controlled blanket, inside a sound attenuating booth.
- ABRs are recorded for the following frequencies: 6, 12, 18, 24, 30 kHz.
Data QC: Once or twice annually, the calibration of the microphone used for sound delivery should be checked using a Bruel and Kjaer PULSE system with the Type 4231 calibrator or other equivalent calibration instrumentation suitable for use with high frequencies.
Measurements
LIMS field name |
MPD variable name |
Description |
Units |
Series |
Primary measurement |
Series order |
MPD computed |
Calculation |
Method |
Data type |
Body weight |
bw |
body weight |
g |
|
|
|
|
|
body weight |
Float |
6kHz-evoked ABR threshold |
pip_6 |
auditory brainstem response (ABR) threshold, sound pressure level, 6 kHz pip |
dB |
Yes |
pip_6 |
1 |
|
|
ABR test |
Float |
12kHz evoked ABR threshold |
pip_12 |
auditory brainstem response (ABR) threshold, sound pressure level, 12 kHz pip |
dB |
Yes |
pip_6 |
2 |
|
|
ABR test |
Float |
18kHz evoked ABR threshold |
pip_18 |
auditory brainstem response (ABR) threshold, sound pressure level, 18 kHz pip |
dB |
Yes |
pip_6 |
3 |
|
|
ABR test |
Float |
24kHz evoked ABR threshold |
pip_24 |
auditory brainstem response (ABR) threshold, sound pressure level, 24 kHz pip |
dB |
Yes |
pip_6 |
4 |
|
|
ABR test |
Float |
30kHz evoked ABR threshold |
pip_30 |
auditory brainstem response (ABR) threshold, sound pressure level, 30 kHz pip |
dB |
Yes |
pip_6 |
5 |
|
|
ABR test |
Float |
Experimental and technical parameters
Equipment name |
Auditory brainstem response system |
Equipment manufacturer |
Intelligent Hearing Systems |
Equipment model |
Smart EP |
Software |
Smart EP |
Recording environment |
Sound proof booth |
Stimulus level |
5 |
Anesthesia |
Ketamine-Xylazine |
|