Hematological parameters in 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Rathkolb B, Wolf E, Lengger C, Kollmus H, Schughart K
Hematology automated analyzer: Sysmex Deutschland GmbH (XT-2000iV)
Reagents
and
solutions
Isoflurane
Procedure: Hematology
Mice (non-fasting) are anesthetized via isoflurane inhalation.
Blood is collected in the morning by retro-orbital puncture where 50 µL of whole blood is collected in an EDTA-coated 50 µL end-to-end capillary, diluted 1:5 with CellPack buffer, carefully mixed and placed on a rotary mixer for a minimun of 30 min.
Samples are kept at room temperature until analyzed.
Analysis is performed on the day of sample collection within 6h after sample collection. Samples with clots are not run.