Bouma1: Gonadal and sex research in a backcross of Dax1 XY mice (2005)

Bouma GJ, Albrecht KH, Washburn LL, Recknagel AK, Churchill GA, Eicher EM. Gonadal sex reversal in mutant Dax1 XY mice: a failure to upregulate Sox9 in pre-Sertoli cells. Development. 2005 Jul;132(13):3045-54.   PubMed 15944188  

Bouma1 downloads
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Participants G J Bouma
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Albrecht KH, Washburn LL, Recknagel AK, Eicher EM
ContactGary A Churchill
AffiliationChurchill Group QTL Archive
AcknowledgementsThis study was supported by NIH grants GM20919 (E.M.E.), HD07065-25 (G.J.B.), GM070683(G.A.C.) and HD042779 (K.H.A.), and NCI core grant CA34196 (The Jackson Laboratory).
Project type QTL Archive data set
MPD identifiersBouma1     MPD:159
Data changelog No updates/corrections.       Initial release date: 04/2010.
The nuclear receptor transcription factor Dax1 is hypothesized to play a role in testicular development. A backcross with mutant Dax1 was generated to identify the function of the gene. 128 male mice were measured for fetal gonads and given different scores based on that. The scores were used to do QTL analysis to test the hypothesis. For detailed protocols see Bouma et al, 2005.

QTL data set parameters:
Progenitor strains B6-Dax1-/+     B6-Dax1+/Y     DBA/2J    
Cross direction notation(B6 Dax1-/+ x D2) F1 x B6-Dax1+/Y
Cross typeBackcross
Population size 0 ♀     148 ♂
Notes148 Dax1-/Y mice

Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Bouma1 15901 microscopy (none) fetal gonads   [designation]  gonads QTL population m None N=148
Bouma1 15902 microscopy (none) scoring of fetal gonadal status   [score]  score QTL population m embryo N=148

Project / data set:   Bouma1     a.k.a. Bouma_2005
Excel file:   Data_Description_BxDbackcross_Bouma2005.xlsx
CSV file:   Bouma2005_BxD_B37_Data.csv
Readme / text file:   readme_Bouma2005.txt
QC plots, figures, etc.:   QCReport_Bouma2005.pdf