Home       About             Help       Projects > Lake1 > Lake1 animal documentation Lake J, Donahue L, Davisson MT Multi-system analysis of mouse physiology, C57BL/6J-Chr#A/J/NaJ chromosome substitution panel Animals were evaluated in these areas: hematology, blood chemistry, lung function, blood pressure and pulse, and electrocardiogram. This project is part of a series, see project page for details. Lake1 project data page       Protocol

Acclimation & testing periods Ages 8-9 wks Feed 6% fat diet (Purina 5K52) Water Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA). Housing Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system. Bedding Steam autoclaved white pine shavings Photoperiod 12 hr on 12 hrs off – 5:00 AM On – 5:00 PM Off Temperature 18 - 24°C Relative humidity ~50% Health status The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.

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