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Project data set: Cheverud1   (2004)

Project Diet-induced obesity and diabetes in 19 LGXSM recombinant inbred strains of mice

There were 2 cohorts: 1) controls on standard diet; and 2) mice fed a high fat diet (42% calories from fat).

Study design: control vs. intervention; 2 cohorts.
19 strains tested     female male     Test age: various

Investigators Cheverud JM   [Details, papers]

Protocol View phenotyping protocol and methods

Cheverud1
measurements



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blood — clinical chemistry         glucose tolerance  
Cheverud1
glucose levels, area under curve, glucose tolerance test
(serum GLU, 10% glucose i.p., 4h fast) [AUC]  
  control and  high-fat diet
        age 10wks:   • control diet    • high-fat diet    • comparison  
        age 20wks:   • control diet    • high-fat diet    • comparison  
        compare ages:   control diet    high-fat diet 
19 strains   ages shown
  LGXSM
 
blood — lipids         lipid profile  
Cheverud1
total cholesterol (serum CHOL) [mg/dL]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
free fatty acid (serum FFA) [mg/dL]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
triglycerides (serum TG) [mg/dL]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
 
body fat pads         scales  
Cheverud1
gonadal fat pad weight [g]     control and  high-fat diet 17wks
        weight [g]:   • control diet    • high-fat diet    • comparison  
        as percent of body weight [%]:   • control diet    • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
perirenal fat pad weight [g]     control and  high-fat diet 17wks
        weight [g]:   • control diet    • high-fat diet    • comparison  
        as percent of body weight [%]:   • control diet    • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
mesenteric fat pad weight [g]     control and  high-fat diet 17wks
        weight [g]:   • control diet    • high-fat diet    • comparison  
        as percent of body weight [%]:   • control diet    • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
inguinal fat pad weight [g]     control and  high-fat diet 17wks
        weight [g]:   • control diet    • high-fat diet    • comparison  
        as percent of body weight [%]:   • control diet    • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
total fat pad weight [g]     control and  high-fat diet 17wks
        weight [g]:   • control diet    • high-fat diet    • comparison  
        as percent of body weight [%]:   • control diet    • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
 
body weight growth curve         scales  
Cheverud1
body weight / growth curve, control diet [g]  
      age (wks): • 4  • 5  • 6  • 7  • 9  • 11  • 15  • 19  • 23    • growth curve comparison  
19 strains   ages shown
  LGXSM
Cheverud1
body weight / growth curve, high-fat diet [g]   high-fat diet  
      age (wks): • 4  • 5  • 6  • 7  • 9  • 11  • 15  • 19  • 23    • growth curve comparison  
19 strains   ages shown
  LGXSM
 
growth rate (weight)         scales  
Cheverud1
pre-weaning (1-3 wks) growth rate [g/wk]  
      • control group  • high-fat diet group 
19 strains   ages shown
  LGXSM
Cheverud1
growth rate [g/wk]     control and  high-fat diet
        post-weaning (4-10wks):   • control diet    • high-fat diet    • comparison  
        adult (11-20wks):   • control diet    • high-fat diet    • comparison  
19 strains   ages shown
  LGXSM
 
cardiovascular — heart weight         scales  
Cheverud1
heart weight as percent of body weight [%]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
 
endocrine — hormones         radioimmunoassay  
Cheverud1
insulin (serum) [ng/mL]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
leptin (serum) [pg/mL]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
 
kidney — morphology — weight         scales  
Cheverud1
kidney weight (both) as percent of body weight [%]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
 
liver — weight         scales  
Cheverud1
liver weight as percent of body weight [%]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
 
spleen — weight         scales  
Cheverud1
spleen weight as percent of body weight [%]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM


local experiment parameter  
Cheverud1
body weight at necropsy [g]     control and  high-fat diet 17wks
      • control diet  • high-fat diet    • comparison  
19 strains   age 20-30wks
  LGXSM
Cheverud1
age at necropsy   [wks]
19 strains  
  Inbred



Number of mice tested per strain, for each measurement

  
Changelog There have been no updates or corrections to the data set since MPD release in Nov 2008  
Reference
Cheverud JM, Ehrich TH, Kenney JP, Pletscher LS, Semenkovich CF. Genetic evidence for discordance between obesity- and diabetes-related traits in the LGXSM recombinant inbred mouse strains. Diabetes. 2004 Oct;53(10):2700-8.     PubMed 15448104     MGI


Hrbek T, de Brito RA, Wang B, Pletscher LS, Cheverud JM. Genetic characterization of a new set of recombinant inbred lines (LGXSM) formed from the inter-cross of SM/J and LG/J inbred mouse strains. Mamm Genome. 2006 May;17(5):417-29.     PubMed 16688532     MGI

Related papers
Mice
Strain and animal info
Note
Genotypic data (Mit markers) for QTL analysis are available in a supplemental file.

Static graphs are available for the glucose clearance tests.

Note
Please note: Low sample sizes (N < 5) are present. [View table]
Note
Some measurements are a mixture of + and – values. [View list]
Note
Some measurements are not eligible for all-MPD pheno vs. pheno
correlations due to data characteristics or semantics [View list]

Cheverud1
downloads


Individual animal data
Table format (.csv)
Individual animal data in table format, with one row per animal and one column per measurement.
Individual animal data
List format (.csv)
Individual animal data organized as one row per animal / measurement reading.
Strain means (.csv) Strain means with SD, N, other summary statistics. One row per strain / sex / measurement.
Supplemental data are available

 See also the MPD download center for more general data download options.





 




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