000461 - B10.D2 Strain Details

Congenic

Major Histocompatibility Congenic

Originating Article

This strain carries the Hc⁰ allele from DBA/2J, making them serum C5 deficient. Mice have increased susceptibility to certain pathogens and impaired chemotactic responses of neutrophils. Allograft rejection is prolonged. The following inbred strains are also homozygous for the Hc⁰ allele: A/HeJ (Stock No. 000645), AKR/J (Stock No. 000648), DBA/2J (Stock No. 000671), NOD/LtJ (Stock No. 001976), NZB/BlNJ (Stock No. 000684), and SWR/J (Stock No. 000689).

This line was developed by Dr. George Snell during the late 1940's and early 1950's from an initial cross between C57BL/10Sn and DBA/2 and progeny were backcrossed for 6 generations to C57BL/10Sn and then maintained by sibling mating. By using tumor rejection for selection each generation, the H2 locus along with the Hc and H2-T18 loci from DBA/2 were selected in this congenic strain. This strain is distinguished from 000460 B10.D2-Hc⁰ H2^d H2-T18^c/o2SnJ by the number of backcross generations.

Genetic Background
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Generation
Generation Definitions >
N6F105p+F6
(Last Updated: May 1st 2025)

Allele Symbol Hc⁰

Allele Name

Gene Symbol

Allele Type

Gene Name

Gene Synonym(s)

Site of Expression

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Expressed Gene(s)

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Strain of Origin

Chromosome

Molecular Note

A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, A/J, AKR/J, DBA/2J, I/LnJ, KK/HlJ, MOLF/EiJ, NZB/B1NJ, RF/J, ST/bJ SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5.

General Note

This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, A/J, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)

Mutations Made By

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Allele Symbol H2^d

Allele Name

Gene Symbol

Allele Type

Gene Name

Gene Synonym(s)

Site of Expression

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Expressed Gene(s)

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Strain of Origin

Chromosome

Molecular Note

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General Note

The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB.

Mutations Made By

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Allele Symbol H2-T18^c

Allele Name

Gene Symbol

Allele Type

Gene Name

Gene Synonym(s)

Site of Expression

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Expressed Gene(s)

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Strain of Origin

Chromosome

Molecular Note

The allele H2-T18^c determines presence of specificities TL.2, 7 and occurs in strains BALB/c, DBA/2, 129/J, and others.

General Note

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Mutations Made By

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B10.D2-Hc⁰ H2^d H2-T18^c/oSnJ

How it's Made