If you need help with your order, contact Customer Support

For in-depth product & services help, ask our Technical Information Scientists
Popular

C57BL/6NJ

What Does This Nomenclature Mean?
Strain #:005304
RRID:IMSR_JAX:005304Info
Common Name: B6N | Also Known As: Black 6N
This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. Five SNP differences have been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ. This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664).
Important Note: This strain is homozygous for Crb1rd8, the retinal degeneration 8 mutation. Photoreceptor degeneration is observed in spots, caused by retinal folds and pseudorosettes, found in the fundus of the eye.
View Pricing
Live MouseLive Mouse
#005304
View as Pricelist
N/A
Male
Female
$3,532.00
Available
Sized to accommodate large orders
Order Now

How it's Made

Strain Highlights
Originating Article
Originating Article
Info When using the B6N mouse strain in a publication, please include JAX stock #005304 in your Materials and Methods section.

Detailed Description
This is an NIH subline of C57BL/6. It was separated from C57BL/6J in 1951. 5 SNP differences have been identified that distinguish C57BL/6NJ from C57BL/6J and C57BL/6ByJ. Both C57BL/6ByJ and C57BL/6NJ type as follows: 08-015199792-M (rs3709624) is C; 11-004367508-M (rs3659787) is A; 13-041017317-M (rs3722313) is C; 15-057561875-M (rs3702158) is G; 19-049914266-M (rs3724876) is T. C57BL/6J types as follows: 08-015199792-M is T; 11-004367508-M is G; 13-041017317-M is T; 15-057561875-M is A; 19-049914266-M is G (Petkov and Wiles 2005.)

This strain does not have the deletion in the Nnt gene that has been found in the C57BL/6J strain (Stock No. 000664). C57BL/6NJ mice are homozygous for Cyfip2M1N, a spontaneous mutation in the cytoplasmic FMR1 interacting protein 2 that results in an amino acid substitution of phenylalanine for serine at position 968 (S968F). The mutation is found in all C57BL/6N substrains but is not present in the C57BL/6J strain or substrains. The mutation results in 45% lower acute response to cocaine as measured by locomotor hyperactivity (Kumar et al., 2013). This strain is also homozygous for the Del(13)1N deletion, common to the NIH sublines, which includes Zfp998 and Zfp997 and has been found to cause the loss of transcriptional repression of non-ecotropic endogenous retroviruses in C57BL/6NJ compared with C57BL/6J, which does not have this deletion (Treger et al., 2019). A 2022 study found vaginal septa in 5.5% of C57BL/6NJ females.

Development
In 1951 C57BL/6J, then at generation F32, were sent from The Jackson Laboratory to The National Institute of Health where they were maintained via sibling mating for decades. In 1980 this subline reached F126 and in 1984 embryos were cryopreserved at the NIH cryopreservation facility. In approximately 1994 some of these embryos were thawed and maintained via sibling mating and this subline was referred to as B6(e84) to specify that it derived from the embryos frozen in 1984. In September and October of 1997 a new set of embryos were cryopreserved from this thawed line by backcrossing +F6 females to their +F5 fathers. In 2005 some of these embryos frozen in 1997 were sent to The Jackson Laboratory where they were thawed and gave rise to C57BL/6NJ. Thus, C57BL/6NJ is devoid of mutations that may have arisen in any C57BL/6N sublines that remained on the shelf after the 1984 freeze. The embryos received by The Jackson Laboratory were thawed, the colony expanded, and re-frozen immediately. This strain is maintained with frequent replenishment from this frozen stock in order to arrest genetic drift.

Genetics
Genetic Background
---
Generation
Generation Definitions >
Contact Technical Support
(Last Updated: August 20th 2021)
Allele Symbol Crb1rd8 ExternalLink
Allele Name
retinal degeneration 8
Gene Symbol
Crb1
Allele Type
Spontaneous
Gene Name
crumbs family member 1, photor... crumbs family member 1, photoreceptor morphogenesis associated
Gene Synonym(s)
7530426H14Rik,A930008G09Rik,CRB1-A, ... 7530426H14Rik, A930008G09Rik, CRB1-A, CRB1-B, CRB1-C, LCA8, RP12,
Site of Expression
---
Expressed Gene(s)
---
Strain of Origin
C57BL/6By or C57BL/6N
Chromosome
1
More
Allele Symbol Cyfip2M1N ExternalLink
Allele Name
mutation 1, National Institute... mutation 1, National Institutes of Health
Gene Symbol
Cyfip2
Allele Type
Spontaneous
Gene Name
cytoplasmic FMR1 interacting p... cytoplasmic FMR1 interacting protein 2
Gene Synonym(s)
1500004I01Rik,6430511D02Rik,DEE65, ... 1500004I01Rik, 6430511D02Rik, DEE65, EIEE65, mKIAA1168, PIR121,
Site of Expression
---
Expressed Gene(s)
---
Strain of Origin
C57BL/6N
Chromosome
11
More
Allele Symbol Nlrp12C57BL/6N ExternalLink
Allele Name
C57BL/6N
Gene Symbol
Nlrp12
Allele Type
Spontaneous
Gene Name
NLR family, pyrin domain conta... NLR family, pyrin domain containing 12
Gene Synonym(s)
CLR19.3,FCAS2,Nalp12, ... CLR19.3, FCAS2, Nalp12, PAN6, PYPAF7, RNO, RNO2,
Site of Expression
---
Expressed Gene(s)
---
Strain of Origin
C57BL/6N
Chromosome
7
More
Allele Symbol Cox7a2ls ExternalLink
Allele Name
short
Gene Symbol
Cox7a2l
Allele Type
Not Applicable
Gene Name
cytochrome c oxidase subunit 7... cytochrome c oxidase subunit 7A2 like
Gene Synonym(s)
COX7AR,COX7RP,EB1, ... COX7AR, COX7RP, EB1, SCAF1, SCAFI, SIG81, SIG-81,
Site of Expression
---
Expressed Gene(s)
---
Strain of Origin
multiple strains
Chromosome
17
More
Allele Symbol Del(13)1N ExternalLink
Allele Name
Deletion, Chr 13, NIH 1
Gene Symbol
Del(13)1N
Allele Type
Spontaneous
Gene Name
Deletion, Chr 13, NIH 1
Gene Synonym(s)
---
Site of Expression
---
Expressed Gene(s)
---
Strain of Origin
C57BL/6N
Chromosome
13
More
Loading...
Loading...