See JAX Pipeline on the IMPC website for order of tasks for all mice tested
Project protocol: Heart weight
Introduction
Using heart weight and body weight to evaluate cardiac size.
Study design
Paradigm: Baseline study
Panel: Collaborative Cross
Number of strains: 18
Sex: Both
Age: 17-19 wks
Sample size: Six mice per sex from at least two litters
Number of cohorts: 1
Workflow
| Step |
Description |
Equipment |
Data collected |
| 1 |
Mice are euthanized |
- |
- |
2 |
Weigh each mouse |
Balance |
Body weight |
| 3 |
Dissect and weigh heart |
Balance |
Heart weight |
Equipment, software and supplies
Reagents and solutions
Procedure
- Mice are euthanized via cervical dislocation.
- Body weight is recorded.
- Each mouse is placed on its back and pinned onto the board (inner side of front and hind paws).
- Each mouse is doused with 70% ethanol to control hair and dander.
- The heart is dissected (dissect aortic root immediately above the aortic valves and the superior vena cava above the atria).
- Mediastinal fat pads are carefully removed with forceps.
- Blood is removed from the heart with Kim wipes or surgical compress until the heart is totally dry.
- The heart is weighed.
Formulas and derivations: Local data processing is implemented to calculate derived measures from these data, and a table of the derived measures and raw data are reported per mouse in JaxLIMS.
Measurements
| LIMS field name |
MPD variable name |
Description |
Units |
Series |
Primary measurement |
Series order |
MPD computed |
Calculation |
Method |
Data type |
| Body weight |
bw |
body weight |
g |
|
|
|
|
|
body weight |
Float |
Heart weight |
heart_wt |
heart weight, weight |
mg |
Yes |
heart_wt |
1 |
|
|
organ weight |
Float |
| HW/BW |
heart_wt_adj |
heart weight, normalized to body weight |
mg |
Yes |
heart_wt |
2 |
|
|
organ weight |
Float |
Experimental and technical parameters
| Equipment name |
Balance |
| Equipment manufacturer |
Ohaus |
| Equipment model |
AV212C |