Geuther1 project protocol

Locomotor activity in 47 inbred strains of mice and 11 F1 hybrids   (2019)

Geuther BQ, Kumar V
With: Deats SP, Fox KJ, Murray SA, Braun RE, White JK, Chesler EJ, Lutz CM

See also: Geuther1 animal documentation


Project protocol - Contents

Workflow and sampling

Step
Procedure
Equipment
Data collected
1
Acclimation in test room (30-45 min) -
-
2 Open field test
Open field arena Locomotor activity

Equipment, software and supplies

  • Open field chamber
    • 52 x 52 x 23 cm
    • Floor is PVC plastic and walls are gray PVC plastic
    • 600 lux
  • Actimetrics LimeLight software

Reagents and solutions

  • Dilute Quatricide solution (for cleaning chamber), Pharmacal Research Labs

Procedure: Open field test

  1. Mice are acclimated to test room for 30-45 min prior to testing.
  2. Activity is measured for 10 min and recorded; mice are allowed to explore freely for a total of 55 min.

Data collected by investigator

  • Distance traveled at 10 min and at 55 min