Diet-induced disease: Gallstones, liver pathology, plasma lipids, and pathogen-accelerated atherosclerosis in obesity mutants.

Available measurements   Mice & strains       Protocol       Plots & tools wizard Change list style       Show varnames       Verbose listing       Display strain data     Compare 2 strains blood chemistry — enzymes   Paigen3   obese mutant alanine aminotransferase (plasma ALT)   [IU/L]   high-fat diet   8wks 8 strains   age 15-19wks   blood chemistry — miscellaneous   Paigen3   obese mutant bile salts (plasma)   [µmol/L]   high-fat diet   8wks 8 strains   age 15-19wks   blood lipids — cholesterol — HDL   Paigen3   obese mutant HDL cholesterol (plasma)   high-fat diet   8wks       • amount        • % of total plasma cholesterol        8 strains   age 15-19wks   blood lipids — cholesterol — non-HDL   Paigen3   obese mutant plasma cholesterol not in the HDL fraction   [mg/dL]   high-fat diet   8wks 8 strains   age 15-19wks   blood lipids — cholesterol — total   Paigen3   obese mutant total cholesterol (plasma)   [mg/dL]   high-fat diet   8wks 8 strains   age 15-19wks   body weight   Paigen3   obese mutant body weight [g]     baseline and  high-fat diet 8wks       • start        • after 8wks on diet        • Δchange after diet          diet effect comparison   8 strains   age 7-11wks                 at baseline   cardiovascular — atherosclerosis   Paigen3   obese mutant fatty streak aortic lesion size   [µm2]   high-fat diet   8wks 8 strains   age 15-19wks   liver and gallbladder — function   Paigen3   obese mutant hepatic cholesterol concentration [mg]   high-fat diet   8wks       • free        • esterified        • total        • free to esterified ratio        8 strains   age 15-19wks   liver and gallbladder — gallbladder pathology and gallstones   Paigen3   obese mutant gallbladder volume   [µL]   high-fat diet   8wks 8 strains   age 15-19wks Paigen3   obese mutant gallbladder pathology (0-4) [score]   high-fat diet   8wks       • mucin        • arc        • filaments        liquid crystals:             • small              • aggregated              • fused        • cholesterol monohydrate crystals        stones:             • sandy              • true              • all              • presence score              8 strains   age 15-19wks   liver and gallbladder — liver morphology   Paigen3   obese mutant liver weight at sacrifice   [g]   high-fat diet   8wks 8 strains   age 15-19wks   liver and gallbladder — liver pathology   Paigen3   obese mutant liver pathology (0-4) [score]   high-fat diet   8wks       • view images and scores        • microvacuoles        • macrovacuoles        • inflammation        8 strains   age 15-19wks   local experiment parameter   Paigen3   obese mutant age at start of diet   [d] 8 strains   age 7-11wks Display strain means in the above table for: select a strain B6(Cg)-Tub<t B6.BKS(D)-Le B6.Cg-A<y>/J B6.V-Lep<ob> BKS.Cg-Dock7 BKSChpLt.HRS C57BL/6J C57BLKS/J   Compare two strains: select strain 1 B6(Cg)-Tub<t B6.BKS(D)-Le B6.Cg-A<y>/J B6.V-Lep<ob> BKS.Cg-Dock7 BKSChpLt.HRS C57BL/6J C57BLKS/J vs. select strain 2 B6(Cg)-Tub<t B6.BKS(D)-Le B6.Cg-A<y>/J B6.V-Lep<ob> BKS.Cg-Dock7 BKSChpLt.HRS C57BL/6J C57BLKS/J   Compare female vs. male for: select a strain B6(Cg)-Tub<t B6.BKS(D)-Le B6.Cg-A<y>/J B6.V-Lep<ob> BKS.Cg-Dock7 BKSChpLt.HRS C57BL/6J C57BLKS/J   Display overall measurement averages (all strains combined) in the above table

Notes

Bouchard G, Johnson D, Carver T, Paigen B, Carey MC. Cholesterol gallstone formation in overweight mice establishes that obesity per se is not linked directly to cholelithiasis risk. J Lipid Res. 2002 Jul;43(7):1105-13.     PubMed    MGI There have been 3 updates or corrections to the dataset since it was first released in Feb, 2001 Mice were housed in a non-barrier facility during the acclimation and testing periods. Three pathogens were detected during this time: Pneumocystis, Pasturella, and Helicobacter. Pathogen-accelerated atherosclerosis was observed in these studies. These results are in contrast to a similar study conducted in a pathogen-free facility (Paigen2). Similar data are available for 43 inbred strains also tested by these investigators; see Paigen1 (identical C57BL/6J and C57BLKS/J data are present in both data sets). This project includes a collection of browsable liver pathology images. Some strain means have N < 5 animals (click for table). Some measurements were derived by MPD staff, based on supplied data (click for list). This is one of several projects supplied by Heart, Lung, Blood and Sleep Disorders Center.

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The Paigen3 project data set. Individual animal data in table format, with one row per animal and measurements organized as columns. Excel file Paigen3 individual animal data in list format. Most or all of the same information* as the file above, but organized as one row per animal / measurement. Excel file Paigen3 strain means, SD, SEM, N, Z-scores and other summary statistics. One row per strain / sex / measurement. Excel file These Excel files include data, descriptions, and units for all measurements listed above. Other download options are available via the Plots & Tools Wizard, above. Paigen3 protocol document HTML Paigen3 animal documentation HTML Supplemental data None