Rating scale to assess ethanol-induced intoxication. Purpose: The goal of this project was to test 9 strains of mice for ethanol effects at four different dosages (2.75, 3.0, 3.25, and 3.5 g of alcohol per kg body weight), and to assess the level of ethanol intoxication based on several parameters including line crossing, wobbling, and splaying behaviors for a duration of 45 seconds in four successive intervals (5, 10, 15, 30 minutes).

Available measurements   Mice & strains       Protocol       Plots & tools wizard Change list style       Show varnames       Verbose listing       Display strain data     Compare 2 strains behavior — activity and motor function &ndash open field test   Metten1 line crossings, 2.75g/kg injection [n]     control and  EtOH 2.75g/kg i.p.       • pre-injection        • 10 min after injection        • 30 min after          time after EtOH comparison   9 strains   age 8-11wks Metten1 line crossings, 3.0g/kg injection [n]     control and  EtOH 3.0g/kg i.p.       • pre-injection        • 10 min after injection        • 30 min after          time after EtOH comparison   9 strains   age 8-11wks Metten1 line crossings, compare dose groups     control and  EtOH       pre-injection        10 min after injection        30 min after        8 strains   age 8-16wks   behavior — activity and motor function — observer-rated ataxia   Metten1 hindlimb splay, 2.75g/kg injection [score]   EtOH   2.75g/kg i.p.       • 10 min after injection        • 30 min after          time after EtOH comparison   9 strains   age 8-11wks Metten1 hindlimb splay, 3.0g/kg injection [score]   EtOH   3.0g/kg i.p.       • 10 min after injection        • 30 min after          time after EtOH comparison   9 strains   age 8-11wks Metten1 erratic gait, 2.75g/kg injection [score]   EtOH   2.75g/kg i.p.       • 10 min after injection        • 30 min after          time after EtOH comparison   9 strains   age 8-11wks Metten1 erratic gait, 3.0g/kg injection [score]   EtOH   3.0g/kg i.p.       • 10 min after injection        • 30 min after          time after EtOH comparison   9 strains   age 8-11wks Metten1 compare dose groups   EtOH         hindlimb splay:             10 min after              30 min after        erratic gait:             10 min after              30 min after              8 strains   age 8-16wks Display strain means in the above table for: select a strain 129P3/J 129S1/SvImJ A/J BALB/cByJ BTBR T+ tf/J C3H/HeJ C57BL/6J DBA/2J FVB/NJ   Compare two strains: select strain 1 129P3/J 129S1/SvImJ A/J BALB/cByJ BTBR T+ tf/J C3H/HeJ C57BL/6J DBA/2J FVB/NJ vs. select strain 2 129P3/J 129S1/SvImJ A/J BALB/cByJ BTBR T+ tf/J C3H/HeJ C57BL/6J DBA/2J FVB/NJ   Compare female vs. male for: select a strain 129P3/J 129S1/SvImJ A/J BALB/cByJ BTBR T+ tf/J C3H/HeJ C57BL/6J DBA/2J FVB/NJ   Display overall measurement averages (all strains combined) in the above table

Notes

Metten P, Best KL, Cameron AJ, Saultz AB, Zuraw JM, Yu CH, Wahlsten D, Crabbe JC. Observer-rated ataxia: Rating scales for assessment of genetic differences in ethanol-induced intoxication in mice. J Appl Physiol. 2004 Jul;97(1):360-8. Epub 2004 Mar 19.     PubMed    MGI There have been no updates or corrections to the dataset since it was first released in Jan, 2007 Please note. Some strain means have N < 3 animals (click for table).

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The Metten1 project data set. Individual animal data in table format, with one row per animal and measurements organized as columns. Excel file Metten1 individual animal data in list format. Most or all of the same information* as the file above, but organized as one row per animal / measurement. Excel file Metten1 strain means, SD, SEM, N, Z-scores and other summary statistics. One row per strain / sex / measurement. Excel file These Excel files include data, descriptions, and units for all measurements listed above. Other download options are available via the Plots & Tools Wizard, above. Metten1 protocol document HTML Metten1 animal documentation HTML Supplemental data are available Click here for more info