Diet effects on obesity and diabetes in LGXSM recombinant inbred strains. Purpose: To investigate phenotypic differences in growth rate, blood chemistry, and body composition, and the effects of high-fat diet in the manifestation of obesity and diabetes in LGXSM recombinant inbred strains.

Available measurements   Mice & strains       Protocol       Plots & tools wizard Change list style       Show varnames       Verbose listing       Display strain data     Compare 2 strains blood chemistry — glucose — glucose tolerance test   Cheverud1   LGXSM glucose levels, area under curve, glucose tolerance test (serum GLU, 10% glucose i.p., 4h fast) [(mg/dL)2]     control and  high-fat diet         age 10wks:   • control diet    • high-fat diet    diet effect comparison           age 20wks:   • control diet    • high-fat diet    diet effect comparison           compare ages:   control diet    high-fat diet  19 strains   ages: see details   blood lipids — cholesterol — total   Cheverud1   LGXSM total cholesterol (serum CHOL) [mg/dL]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   blood lipids — fatty acids   Cheverud1   LGXSM free fatty acid (serum FFA) [mg/dL]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   blood lipids — triglycerides   Cheverud1   LGXSM triglycerides (serum TG) [mg/dL]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   body composition — fat pads   Cheverud1   LGXSM fat pad weight [g]     control and  high-fat diet 17wks         gonadal:   • control diet    • high-fat diet    diet effect comparison           perirenal:   • control diet    • high-fat diet    diet effect comparison           mesenteric:   • control diet    • high-fat diet    diet effect comparison           inguinal:   • control diet    • high-fat diet    diet effect comparison           total:   • control diet    • high-fat diet    diet effect comparison   19 strains   age 20-30wks Cheverud1   LGXSM fat pad weight as % of body weight [%]     control and  high-fat diet 17wks         gonadal:   • control diet    • high-fat diet    diet effect comparison           perirenal:   • control diet    • high-fat diet    diet effect comparison           mesenteric:   • control diet    • high-fat diet    diet effect comparison           inguinal:   • control diet    • high-fat diet    diet effect comparison           total:   • control diet    • high-fat diet    diet effect comparison   19 strains   age 20-30wks   body weight growth curve   Cheverud1   LGXSM body weight / growth curve, control diet [g]         age (wks): • 4  • 5  • 6  • 7  • 9  • 11  • 15  • 19  • 23    growth curve comparison   19 strains   ages: see details Cheverud1   LGXSM body weight / growth curve, high-fat diet [g]   high-fat diet         age (wks): • 4  • 5  • 6  • 7  • 9  • 11  • 15  • 19  • 23    growth curve comparison   19 strains   ages: see details   growth rate (weight)   Cheverud1   LGXSM pre-weaning (1-3wks) growth rate [g/wk]         • control group  • high-fat diet group  19 strains   ages: see details Cheverud1   LGXSM growth rate [g/wk]     control and  high-fat diet         post-weaning (4-10wks):   • control diet    • high-fat diet    diet effect comparison           adult (11-20wks):   • control diet    • high-fat diet    diet effect comparison   19 strains   ages: see details   cardiovascular — morphology — organ weight   Cheverud1   LGXSM heart weight as % of body weight [%]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   endocrine — hormones   Cheverud1   LGXSM insulin (serum) [ng/mL]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks Cheverud1   LGXSM leptin (serum) [pg/mL]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   kidney — morphology — metrics   Cheverud1   LGXSM kidney weight (both) as % of body weight [%]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   liver and gallbladder — liver morphology   Cheverud1   LGXSM liver weight as % of body weight [%]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   local experiment parameter   Cheverud1   LGXSM body weight at necropsy [g]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks   spleen — morphology — organ weight   Cheverud1   LGXSM spleen weight as % of body weight [%]     control and  high-fat diet 17wks       • control diet  • high-fat diet    diet effect comparison   19 strains   age 20-30wks Display strain means in the above table for: select a strain LG/J LGXSM10 LGXSM15 LGXSM18 LGXSM19 LGXSM20 LGXSM22 LGXSM23 LGXSM31 LGXSM33 LGXSM35 LGXSM38 LGXSM39 LGXSM4 LGXSM45 LGXSM46 LGXSM48 LGXSM5 SM/J   Compare two strains: select strain 1 LG/J LGXSM10 LGXSM15 LGXSM18 LGXSM19 LGXSM20 LGXSM22 LGXSM23 LGXSM31 LGXSM33 LGXSM35 LGXSM38 LGXSM39 LGXSM4 LGXSM45 LGXSM46 LGXSM48 LGXSM5 SM/J vs. select strain 2 LG/J LGXSM10 LGXSM15 LGXSM18 LGXSM19 LGXSM20 LGXSM22 LGXSM23 LGXSM31 LGXSM33 LGXSM35 LGXSM38 LGXSM39 LGXSM4 LGXSM45 LGXSM46 LGXSM48 LGXSM5 SM/J   Compare female vs. male for: select a strain LG/J LGXSM10 LGXSM15 LGXSM18 LGXSM19 LGXSM20 LGXSM22 LGXSM23 LGXSM31 LGXSM33 LGXSM35 LGXSM38 LGXSM39 LGXSM4 LGXSM45 LGXSM46 LGXSM48 LGXSM5 SM/J   Display overall measurement averages (all strains combined) in the above table

Notes

Cheverud JM, Ehrich TH, Kenney JP, Pletscher LS, Semenkovich CF. Genetic evidence for discordance between obesity- and diabetes-related traits in the LGXSM recombinant inbred mouse strains. Diabetes. 2004 Oct;53(10):2700-8.     PubMed    MGI Fawcett GL, Roseman CC, Jarvis JP, Wang B, Wolf JB, Cheverud JM. Genetic architecture of adiposity and organ weight using combined generation QTL analysis. Obesity (Silver Spring). 2008 Aug;16(8):1861-8. Epub 2008 Jun 12.     PubMed    MGI Hrbek T, de Brito RA, Wang B, Pletscher LS, Cheverud JM. Genetic characterization of a new set of recombinant inbred lines (LGXSM) formed from the inter-cross of SM/J and LG/J inbred mouse strains. Mamm Genome. 2006 May;17(5):417-29.     PubMed    MGI Reich MS, Jarvis JP, Silva MJ, Cheverud JM. Genetic relationships between obesity and osteoporosis in LGXSM recombinant inbred mice. Genet Res. 2008 Oct;90(5):433-44.     PubMed    MGI There have been no updates or corrections to the dataset since it was first released in Nov, 2008 Genotypic data (Mit markers) for QTL analysis are available in a supplemental file. Static graphs are available for the glucose clearance tests. Some strain means have N < 5 animals (click for table). Some measurements include a mixture of positive and negative values. (click for list). See also the investigators' web site.

Downloads             Help

The Cheverud1 project data set. Individual animal data in table format, with one row per animal and measurements organized as columns. Excel file Cheverud1 individual animal data in list format. Most or all of the same information* as the file above, but organized as one row per animal / measurement. Excel file Cheverud1 strain means, SD, SEM, N, Z-scores and other summary statistics. One row per strain / sex / measurement. Excel file These Excel files include data, descriptions, and units for all measurements listed above. Other download options are available via the Plots & Tools Wizard, above. Cheverud1 protocol document HTML Cheverud1 animal documentation HTML Supplemental data are available Click here for more info