Rusyn6: Trichloroethylene metabolism and tissue-specific toxicity in males of 7 inbred strains of mice (2014)

Yoo HS, Bradford BU, Kosyk O, Shymonyak S, Uehara T, Collins LB, Bodnar WM, Ball LM, Gold A, Rusyn I. Comparative analysis of the relationship between trichloroethylene metabolism and tissue-specific toxicity among inbred mouse strains: liver effects. J Toxicol Environ Health A. 2015;78(1):15-3doi: 10.1080/15287394.2015.958417.   PubMed 25424544     FullText

Yoo HS, Bradford BU, Kosyk O, Uehara T, Shymonyak S, Collins LB, Bodnar WM, Ball LM, Gold A, Rusyn I. Comparative analysis of the relationship between trichloroethylene metabolism and tissue-specific toxicity among inbred mouse strains: kidney effects. J Toxicol Environ Health A. 2015;78(1):32-49. doi: 10.1080/15287394.2015.958418.   PubMed 25424545     FullText


       
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Participants Ivan Rusyn       Texas A&M University College Station, TX
With: Yoo HS, Bradford BU, Kosyk O, Shymonyak S, Uehara T, Collins LB, Bodnar WM, Ball LM, Gold A
ContactIvan Rusyn     IRusyn@cvm.tamu.edu     Lab web site
AcknowledgementsFunding provided by NIH ES005948, ES010126; AstraZeneca
Project type Phenotype strain survey data set
MPD identifiersRusyn6     MPD:512
Data changelog No updates/corrections.       Initial release date: 06/2014.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
A population-based approach was used to test the hypothesis that trichloroethylene (TCE) induced toxicity is associated with the formation of oxidative TCE metabolites. Liver, kidney and serum samples were tested. Major metabolizing enzymes responsible for oxidative biotransformation of TCE, as well as markers of liver and kidney cell proliferation and kidney damage were examined.

Summary of procedures that were conducted
• organ weights Liver, kidney weights. Trichloroethylene-exposed vs. control.
• metabolic panel Blood urea nitrogen (serum). Trichloroethylene-exposed vs. control.
• drug and metabolite quantification Trichloroethylene metabolite in blood, liver, kidney after trichloroethylene exposure.
• RNA expression profiling Trichloroethylene effect on mRNA gene expression in liver and kidney, relative to controls.
• histopathology Kidney histopathology evaluation after trichloroethylene exposure, relative to controls.
Mice: inbred   7 strains   ♂   age 6-7wks   2 cohorts

Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Rusyn6 51202 drug and metabolite quantification trichloroethylene trichloroacetic acid (serum TCA)   [nmol/mL]  TCA_serum_tce inbred 7 m 6-7wks N=3
    Rusyn6 51204 drug and metabolite quantification trichloroethylene dichloroacetic acid (serum DCA)   [nmol/mL]  DCA_serum_tce inbred 7 m 6-7wks N=3
    Rusyn6 51206 drug and metabolite quantification trichloroethylene S-(1,2-dichlorovinyl) glutathione (serum DCVG)   [pmol/mL]  DCVG_serum_tce inbred 4 m 6-7wks N=2
    Rusyn6 51208 drug and metabolite quantification trichloroethylene S-(1,2-dichlorovinyl)-L-cysteine (serum DCVC)   [pmol/mL]  DCVC_serum_tce inbred 7 m 6-7wks N=3
    Rusyn6 51209 metabolic panel trichloroethylene blood urea nitrogen (serum BUN)   [mg/dL]     control    TCE inbred 7 m 6-7wks N=4
    Rusyn6 51211 organ weights trichloroethylene liver weight as a percent of body weight   [%]     control    TCE inbred 7 m 6-7wks N=4
    Rusyn6 51214 histopathology trichloroethylene BrdU-positive nuclei in liver (hepatocellular proliferation), relative to control group   [%]  BrdU_liver_tce inbred 7 m 6-7wks N=3
    Rusyn6 51216 drug and metabolite quantification trichloroethylene trichloroacetic acid (liver TCA)   [nmol/g]  TCA_liver_tce inbred 7 m 6-7wks N=4
    Rusyn6 51218 drug and metabolite quantification trichloroethylene dichloroacetic acid (liver DCA)   [nmol/g]  DCA_liver_tce inbred 7 m 6-7wks N=4
    Rusyn6 51220 drug and metabolite quantification trichloroethylene trichloroethanol (liver TCOH)   [nmol/g]  TCOH_liver_tce inbred 7 m 6-7wks N=4
    Rusyn6 51222 drug and metabolite quantification trichloroethylene S-(1,2-dichlorovinyl) glutathione (liver DCVG)   [pmol/g]  DCVG_liver_tce inbred 7 m 6-7wks N=4
    Rusyn6 51226 RNA expression profiling trichloroethylene Acox1, mRNA in liver, relative to control group   [ratio]  Acox1_liver_tce inbred 7 m 6-7wks N=3
    Rusyn6 51228 RNA expression profiling trichloroethylene Cyp4a10, mRNA in liver, relative to control group   [ratio]  Cyp4a10_liver_tce inbred 7 m 6-7wks N=3
    Rusyn6 51230 RNA expression profiling trichloroethylene Ppara, mRNA in liver, relative to control group   [ratio]  Ppara_liver_tce inbred 7 m 6-7wks N=3
    Rusyn6 51251 organ weights trichloroethylene kidney weight as a percent of body weight   [%]     control    TCE inbred 7 m 6-7wks N=4
    Rusyn6 51254 histopathology trichloroethylene BrdU-positive nuclei in kidney (proximal tubule cell proliferation), relative to control group   [%]  BrdU_kidney_tce inbred 7 m 6-7wks N=3
    Rusyn6 51255 histopathology trichloroethylene kidney injury molecule 1 (KIM-1) positive proximal tubules   [%]     control    TCE inbred 7 m 6-7wks N=4
    Rusyn6 51258 drug and metabolite quantification trichloroethylene trichloroacetic acid (kidney TCA)   [nmol/g]  TCA_kidney_tce inbred 7 m 6-7wks N=4
    Rusyn6 51260 drug and metabolite quantification trichloroethylene dichloroacetic acid (kidney DCA)   [nmol/g]  DCA_kidney_tce inbred 6 m 6-7wks N=3
    Rusyn6 51262 drug and metabolite quantification trichloroethylene trichloroethanol (kidney TCOH)   [nmol/g]  TCOH_kidney_tce inbred 7 m 6-7wks N=4
    Rusyn6 51264 drug and metabolite quantification trichloroethylene S-(1,2-dichlorovinyl) glutathione (kidney DCVG)   [pmol/g]  DCVG_kidney_tce inbred 6 m 6-7wks N=3
    Rusyn6 51266 drug and metabolite quantification trichloroethylene S-(1,2-dichlorovinyl)-L-cysteine (kidney DCVC)   [pmol/g]  DCVC_kidney_tce inbred 6 m 6-7wks N=3
    Rusyn6 51268 RNA expression profiling trichloroethylene Acox1, mRNA in kidney, relative to control group   [ratio]  Acox1_kidney_tce inbred 7 m 6-7wks N=3
    Rusyn6 51270 RNA expression profiling trichloroethylene Cyp4a10, mRNA in kidney, relative to control group   [ratio]  Cyp4a10_kidney_tce inbred 7 m 6-7wks N=3
    Rusyn6 51272 RNA expression profiling trichloroethylene Ppara, mRNA in kidney, relative to control group   [ratio]  Ppara_kidney_tce inbred 7 m 6-7wks N=3
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