Rusyn5:
Drug study: High-fat diet plus subchronic alcohol-induced effects in males of 14 inbred strains of mice (2011)
Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Rusyn I. Interstrain differences in liver injury and one-carbon metabolism in alcohol-fed mice.
Hepatology. 2012 Jul;56(1):130-9. doi: 10.1002/hep.2564Epub 2012 Jun 6.
PubMed 22307928FullText
Ivan Rusyn Texas A&M University, College Station, TX
Participants
Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Gatti DM, Kawaguchi A, Lam TC, Uehara T, Wright FA
Click above to copy-paste the entire citation for this MPD web page.
The effects of high-fat diet on alcohol-induced liver injury were assessed using an intragastric intubation model. Urine alcohol content was monitored daily. Mice were evaluated for endoplasmic reticulum stress response as well as lipid and one-carbon metabolism. There were 2 cohorts: (1) mice fed high-fat diet only; (2) mice fed high-fat diet plus alcohol.
Procedures conducted:
• metabolic panel
Alanine transaminase (serum) after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• alcohol quantification
Alcohol concentration in blood and urine after 4 wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• organ weights
Liver weight after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• histopathology
Liver histopathology, injury after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• metabolite quantification
Homocysteine concentration after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• lipid profile
Hepatic triglycerides after 4wk regimen of ethanol and high-fat diet. 24h fast.
• RNA expression profiling
Liver gene expression after 4wk regimen of ethanol and high-fat diet. 24h fast. mRNA abundance relative to the control (high-fat only) group.
Mice: inbred
14 strains
♂
age 8-14wks
2 experimental groups