Rusyn5: Drug study: High-fat diet plus subchronic alcohol-induced effects in males of 14 inbred strains of mice (2011)

Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Rusyn I. Interstrain differences in liver injury and one-carbon metabolism in alcohol-fed mice. Hepatology. 2012 Jul;56(1):130-9. doi: 10.1002/hep.2564Epub 2012 Jun 6.   PubMed 22307928     FullText


       
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Participants Ivan Rusyn       Texas A&M University College Station, TX
With: Tsuchiya M, Ji C, Kosyk O, Shymonyak S, Melnyk S, Kono H, Tryndyak V, Muskhelishvili L, Pogribny IP, Kaplowitz N, Gatti DM, Kawaguchi A, Lam TC, Uehara T, Wright FA
ContactIvan Rusyn     IRusyn@cvm.tamu.edu     Lab web site
AcknowledgementsFunding provided by NIH AA016258, AA014428, AA018846, ES010126, ES015241
Project type Phenotype strain survey data set
MPD identifiersRusyn5     MPD:430
Data changelog 2 updates/corrections.       Initial release date: 07/2012.
Formatted citation
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The effects of high-fat diet on alcohol-induced liver injury were assessed using an intragastric intubation model. Urine alcohol content was monitored daily. Mice were evaluated for endoplasmic reticulum stress response as well as lipid and one-carbon metabolism. There were 2 cohorts: (1) mice fed high-fat diet only; (2) mice fed high-fat diet plus alcohol.

Summary of procedures that were conducted
• metabolic panel Alanine transaminase (serum) after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• alcohol quantification Alcohol concentration in blood and urine after 4 wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• organ weights Liver weight after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• histopathology Liver histopathology, injury after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• metabolite quantification Homocysteine concentration after 4wk regimen of ethanol vs. control, both groups on high-fat diet. 24h fast.
• lipid profile Hepatic triglycerides after 4wk regimen of ethanol and high-fat diet. 24h fast.
• RNA expression profiling Liver gene expression after 4wk regimen of ethanol and high-fat diet. 24h fast. mRNA abundance relative to the control (high-fat only) group.
Mice: inbred   14 strains   ♂   age 8-14wks   2 cohorts

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Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Rusyn5 43001 metabolic panel high fat diet and ethanol serum alanine transaminase (ALT)   [IU/L]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43003 metabolite quantification high fat diet and ethanol homocysteine concentration (plasma)   [nmol/mL]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43005 alcohol quantification high fat diet and ethanol blood alcohol concentration (BAC)   [mg/dL]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43007 alcohol quantification high fat diet and ethanol urine alcohol concentration (UAC), daily average   [mg/dL]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43009 alcohol quantification high fat diet and ethanol urine alcohol concentration (UAC), daily peak   [mg/dL]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43011 organ weights high fat diet and ethanol liver weight as a percent of body weight   [%]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43013 histopathology high fat diet and ethanol liver steatosis (0-4)   [score]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43015 histopathology high fat diet and ethanol liver inflammation (0-2)   [score]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43017 histopathology high fat diet and ethanol liver necrosis (0-2)   [score]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43019 histopathology high fat diet and ethanol total liver histopathology (0-8)   [score]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43021 lipid profile high fat diet and ethanol hepatic triglycerides (TG)   [mg/g]     high-fat only    high-fat and ethanol inbred 6 m 8-14wks N=3
    Rusyn5 43023 metabolite quantification high fat diet and ethanol reduced liver glutathione (GSH)   [nmol/mg]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43025 metabolite quantification high fat diet and ethanol oxidized liver glutathione (GSSG)   [nmol/mg]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43029 metabolite quantification high fat diet and ethanol ratio of reduced to oxidized liver glutathione   [ratio]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43031 metabolite quantification high fat diet and ethanol liver homocysteine   [nmol/mg]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43033 metabolite quantification high fat diet and ethanol liver S-adenosylhomocysteine (SAH)   [nmol/mg]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43035 metabolite quantification high fat diet and ethanol liver methionine   [nmol/mg]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43037 metabolite quantification high fat diet and ethanol liver S-adenosylmethionine (SAM)   [nmol/mg]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43039 metabolite quantification high fat diet and ethanol ratio of liver SAM to SAH (SAM/SAH)   [ratio]     high-fat only    high-fat and ethanol inbred 14 m 8-14wks N=3
    Rusyn5 43042 metabolite quantification high fat diet and ethanol  4wks BHMT, protein expression relative to high-fat-only group   [%]  liver_BHMT_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43044 metabolite quantification high fat diet and ethanol  4wks DDIT3 (formerly CHOP), protein expression relative to high-fat-only group   [%]  liver_DDIT3_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43046 metabolite quantification high fat diet and ethanol  4wks HSPA5 (formerly GRP78), protein expression relative to high-fat-only group   [%]  liver_HSPA5_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43048 metabolite quantification high fat diet and ethanol  4wks SREBF1 (formerly NSREBP1), protein expression relative to high-fat-only group   [%]  liver_SREBF1_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43050 RNA expression profiling high fat diet and ethanol  4wks Acox1, mRNA abundance relative to high-fat-only group   [%]  liver_Acox1_hfe inbred 6 m 8-14wks N=3
    Rusyn5 43052 RNA expression profiling high fat diet and ethanol  4wks Adipor2, mRNA abundance relative to high-fat-only group   [%]  liver_Adipor2_hfe inbred 6 m 8-14wks N=3
    Rusyn5 43054 RNA expression profiling high fat diet and ethanol  4wks Ahcy, mRNA abundance relative to high-fat-only group   [%]  liver_Ahcy_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43056 RNA expression profiling high fat diet and ethanol  4wks Cbs, mRNA abundance relative to high-fat-only group   [%]  liver_Cbs_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43058 RNA expression profiling high fat diet and ethanol  4wks Cebpa, mRNA abundance relative to high-fat-only group   [%]  liver_Cebpa_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43060 RNA expression profiling high fat diet and ethanol  4wks Cth, mRNA abundance relative to high-fat-only group   [%]  liver_Cth_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43062 RNA expression profiling high fat diet and ethanol  4wks Ddit3 (formerly Chop), mRNA abundance relative to high-fat-only group   [%]  liver_Ddit3_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43064 RNA expression profiling high fat diet and ethanol  4wks Gnmt, mRNA abundance relative to high-fat-only group   [%]  liver_Gnmt_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43066 RNA expression profiling high fat diet and ethanol  4wks Mat1a, mRNA abundance relative to high-fat-only group   [%]  liver_Mat1a_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43068 RNA expression profiling high fat diet and ethanol  4wks Mthfr, mRNA abundance relative to high-fat-only group   [%]  liver_Mthfr_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43070 RNA expression profiling high fat diet and ethanol  4wks Mtr, mRNA abundance relative to high-fat-only group   [%]  liver_Mtr_hfe inbred 14 m 8-14wks N=3
    Rusyn5 43072 RNA expression profiling high fat diet and ethanol  4wks Mttp, mRNA abundance relative to high-fat-only group   [%]  liver_Mttp_hfe inbred 6 m 8-14wks N=3
    Rusyn5 43074 RNA expression profiling high fat diet and ethanol  4wks Srebf1, mRNA abundance relative to high-fat-only group   [%]  liver_Srebf1_hfe inbred 14 m 8-14wks N=3
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