Reed2 project protocol

Bone, body composition and taste phenotypes in Monell consomic strains 129P3/J-Chr#C57BL/6ByJ/MonMmjax and C57BL/6ByJ-Chr#129P3/J/MonMmjax   (2015)

Reed DR, Bachmanov AA
With: Lin C, Fesi B, Arayata C

See also: Reed2 animal documentation


Project protocol - Contents

Workflow and sampling

Step
Procedure
Equipment
Data collected
1
Mice euthanized, weighed and measured Scales, ruler, and electronic calipers
Body weight, length
2
Organs and fat pads harvested and weighed Scales
Weights of selected organs and fat pads
3 Dual-energy X-ray absorptiometry (DXA) Densiotometer (Lunar PIXImus II) Body composition, bone area, bone mineral content and density

Equipment and supplies

  • Scales
  • Electronic calipers (Fowler ProMax, Kelley and Kelley Industrial Supply, Syracuse NY)
  • Lunar PIXImus II densiotometer (GE, software v 2.0., Lunar Corp, Madison WI)

Procedure: Body weight and length, organ weights

    1. Mice are euthanized and weighed using scales.
    2. Mice are measured for length using either a ruler or electronic calipers (bottom of incisors to anus).
    3. Organs are harvested and weighed (brain, heart, kidneys, liver, pancreas, spleen).

Procedure: Body composition and bone mineral content and density

    1. Mice are subjected to DXA (head excluded).
    2. Bone area, fat and lean weights, bone minerals, and bone mineral density are measured.

Data collected by investigator

  • Body weight and length
  • Organ weights
  • Fat pad weights
  • Body composition
  • Bone area
  • Bone mineral content and density