Nadeau1 project protocol

Diet-induced obesity survey in C57BL/6J-Chr#^A/J/NaJ mouse chromosome substitution panel (males)   (2008)

Nadeau JH, Hill AE

Nadeau1 animal documentation

Project protocol — Contents
Workflow and sampling
Equipment
Reagents, supplies, and solutions
Procedure
Data
References

Workflow and sampling

Workflow

Test	Procedure performed	Diet	Age (wk)	Data collected
1	Mice are weaned	regular chow	4	-
2	Baseline body weights are obtained before high fat and high sucrose diet is begun	HFHS	5	baseline body weight
3	After 12 wks on HFHS diet, the final weights of the mice are obtained	HFHS	17	final body weight

Equipment

Top loading balance, TR-402 model, Denver Instrument Company

Reagents, supplies, solutions

Surwit’s Rodent Diet, D12331

HFHS Diet	%/gm	% kcal	g/Kg	kcal
Fat	35.8	58	358	3224.1
Carbohydrates	35.5	25.5	355	1417.4
Protein	23.0	16.4	230	911.6
Sucrose	17.5	12.6	175	700
Cornstarch	0	0	0	0
Coconut Oil, Hydrogenated	33.35	54	333.5	3001.5
Soybean Oil	2.5	4	25	225

Acclimation to test conditions

The mice are weighed at weekly intervals in the mouse room.

Procedure

a. Mice are weighed (baseline) and administered a HFHS diet at 5 wks of age ad libitum.
b. After 12 wks on HFHS diet, mice are weighed a final time (at 17 wks of age).

Data submitted by investigator

Initial body weight and final body weight.