Nadeau1 project protocol

Diet-induced obesity survey in C57BL/6J-Chr#A/J/NaJ mouse chromosome substitution panel (males)   (2008)

Nadeau JH, Hill AE

See also: Nadeau1 animal documentation


Nadeau1_Protocol

Project protocol — Contents
Workflow and sampling
Equipment
Reagents, supplies, and solutions
Procedure
Data
References



Workflow and sampling

Workflow

Test
Procedure performed
Diet
Age (wk)
Data collected
1
Mice are weaned
regular chow
4
-
2
Baseline body weights are obtained before high fat and high sucrose diet is begun
HFHS
5
baseline body weight
3
After 12 wks on HFHS diet, the final weights of the mice are obtained
HFHS
17
final body weight

Equipment

Top loading balance, TR-402 model, Denver Instrument Company

Reagents, supplies, solutions

Surwit’s Rodent Diet, D12331

HFHS Diet
%/gm
% kcal
g/Kg
kcal
Fat
35.8
58
358
3224.1
Carbohydrates
35.5
25.5
355
1417.4
Protein
23.0
16.4
230
911.6
Sucrose
17.5
12.6
175
700
Cornstarch
0
0
0
0
Coconut Oil, Hydrogenated
33.35
54
333.5
3001.5
Soybean Oil
2.5
4
25
225

Acclimation to test conditions

The mice are weighed at weekly intervals in the mouse room.

Procedure

a. Mice are weighed (baseline) and administered a HFHS diet at 5 wks of age ad libitum.
b. After 12 wks on HFHS diet, mice are weighed a final time (at 17 wks of age).

Data submitted by investigator

Initial body weight and final body weight.