Morgan1 project protocol
Drug study: Effects of olanzapine on body weight in 9 inbred strains of mice (2014)
Morgan AP, Crowley JJ, Sullivan PF
With: Nonneman RJ, Quackenbush CR, Miller CN, Ryan AK, Bogue MA, Paredes SH, Yourstone S, Carroll IM, Kawula TH, Bower MA, Sartor RB
Project
protocol
-
Contents
Workflow
and
sampling
Step |
Procedure |
Equipment |
Data
collected
* |
1 |
Body
weight
measured
at
7
wks
of
age
(pre-treatment) |
Scales |
Body
weight |
2 |
Mice
placed
on
high-fat
diet
with
or
without
olanzapine
at
8
wks
of
age |
- |
- |
| 3 |
Mice
weighed
weekly
from
8-18
wks
of
age |
Scales |
Body
weight |
*Supplementary
data
are
available
for
this
project.
See
Morgan1
Procedure:
Olanzapine
preparation
and
administration
- Olanzapine
is
compounded
into
high-fat
food
at
a
concentration
of
50
mg/kg
of
diet
by
Research
Diets,
Inc.
(D0909293;
45
kcal%
fat).
- Control
animals
receive
the
same
high-fat
diet,
minus
olanzapine,
prepared
in
parallel
to
control
for
batch
effects.
Procedure:
Body
weights
- Mice
are
weighed
weekly,
starting
at
7
wks
of
age
(pre-treatment).
Weight
at
8
wks
of
age
is
considered
baseline
for
percent
change
in
body
weight.
Data
collected
by
investigator
- body
weight
(control
and
treated
mice)
Percent
change
in
body
weight
per
animal
computed
by
MPD
*Supplemental
data
(body
weights
at
7-18
wks)
are
available
for
this
project.
See
Morgan1
|