Massett1 project protocol

Exercise capacity in 34 inbred strains of mice   (2012)

Massett MP
With: Courtney SM

See also: Massett1 animal documentation


Massett1_protocol

Project protocol - Contents

Workflow and sampling

Step
Procedure performed
Equipment
Data collected
1
Familiarization with equipment
treadmill
-
2
Body weight measured prior to each test
scale
body weight
3
Endurance exercise test
treadmill
duration, distance, work

Equipment and supplies

  • scales
  • six-lane treadmill (Columbus Instruments, Columbus OH)

Reagents and solutions

  • cleaning solution

Acclimation to test conditions

Mice were allowed one week to become accustomed to the housing facility before starting the exercise test.

Procedure: Endurance exercise test

Mice perform two graded exercise endurance tests on a 6-lane treadmill, separated by 48 h. For each mouse, the test average is taken.

    1. Mice are first familiarized for 2 days: day 1 running for 10 min, 10° incline, at a speed of 9 m/min; day 2 running for 10 min, 10° incline, at a speed of 11 m/min.
    2. Mice are tested on days 3 and 5 after weighing. The exercise capacity protocol is as follows: 9 min, 0° incline, speed 9 m/min --> 9 min, 5° incline, speed 10 m/min --> thereafter speed was increased 2.5 m/min every 3 min, grade was increased 5° every 9 min to a maximum grade of 15°.
    3. Testing is terminated when mice maintain contact with the grid for more than 15 s continuously or can no longer be motivated to run.

Definitions and calculations

  • work: calculated as a product of body weight and vertical distance, where vertical distance = distance run x sin(theta), where theta is equal to the angle of the treadmill

Data collected by investigator

  • body weight
  • duration
  • distance
  • work