Mahler1: Subphenotypes of colitis susceptibility in IL-10-deficient mice generated from backcrosses involving C3H/HeJBir and C57BL/6J (2002)

Mahler M, Most C, Schmidtke S, Sundberg JP, Li R, Hedrich HJ, Churchill GA. Genetics of colitis susceptibility in IL-10-deficient mice: backcross versus F2 results contrasted by principal component analysis. Genomics. 2002 Sep;80(3):274-82.   PubMed 12213197  


     
Mahler1 downloads
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Use the QTL Data Downloads button near the bottom of this page.
Participants M Mahler
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Most C, Schmidtke S, Sundberg JP, Li R, Hedrich HJ
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsFunding provided by NIH DK44240, CA34196; and the Deutsche Forschungsgemeinschaft
Project type QTL Archive data set
MPD identifiersMahler1     MPD:121
Data changelog No updates/corrections.       Initial release date: 01/2008.
Backcrosses involving C3H/HeJBir and C57BL/6J inbred mouse strains homozygous for the disrupted Il10 gene were generated to identify QTLs involved in colitis susceptibility. Spleens were assessed in backcross progeny and the cecum and colon were scored for lesions based on severity, ulceration, hyperplasia, and percentage of area involved. For detailed protocols see Mahler et al 2002.


QTL data set parameters:
Cross 1: (C3H x B6) x B6 backcross       Mahler1
Progenitor strains B6.Il10-/-     C3H.Il10-/-    
Cross direction notation(C3H x B6) x B6
Cross typeBackcross
Population size 100 ♀     103 ♂
NotesN2 progeny
Age6wks

Cross 2: (C3H x B6) x C3H backcross       Mahler1b
Progenitor strains B6.Il10-/-     C3H.Il10-/-    
Cross direction notation(C3H x B6) x C3H
Cross typeBackcross
Population size 116 ♀     143 ♂
Age6wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Mahler1 12101 organ weights (none) spleen to body weight ratio, first Principal Component   [ratio]  SplBW QTL population both 6wks N=102
Mahler1 12102 histopathology (none) cecum assessment, first Principal Component  CecumPC1 QTL population both 6wks N=102
Mahler1 12103 histopathology (none) proximal colon assessment, first Principal Component  ProxPC1 QTL population both 6wks N=102
Mahler1 12104 histopathology (none) middle colon assessment, first Principal Component  MidPC1 QTL population both 6wks N=102
Mahler1 12105 histopathology (none) distal colon assessment, first Principal Component  DistPC1 QTL population both 6wks N=102
Mahler1 12106 histopathology (none) ulceration (score 0-4, increasing severity), first Principal Component  Ulceration-PC1 QTL population both 6wks N=102
Mahler1 12107 histopathology (none) ulceration (score 0-4, increasing severity), second Principal Component  Ulceration-PC2 QTL population both 6wks N=102

Associated genomic features
MGI-curated genes / genomic features that have been associated with this data set:
Cdcs10   Cdcs7   Cdcs8   Cdcs9  


Cross 1: (C3H x B6) x B6 backcross     Mahler1     a.k.a. Mahler_2002a
Excel file:   Data_Description_C3HxB6_Mahler2002a.xlsx
CSV file:   Mahler2002a_B6BC_B37_Data.csv
Readme / text file:   readme_Mahler2002a.txt
QC plots, figures, etc.:   QCReport_Mahler2002a.pdf

Cross 2: (C3H x B6) x C3H backcross     Mahler1b     a.k.a. Mahler_2002b
Excel file:   Data_Description_C3HxB6_Mahler2002b.xlsx
CSV file:   Mahler2002b_C3HBC_B37_Data.csv
Readme / text file:   readme_Mahler2002b.txt
QC plots, figures, etc.:   QCReport_Mahler2002b.pdf