Crabbe1: Drug study: Evaluation of ethanol sensitivity and intoxication using motor incoordination in the balance beam and grid test, in 8 inbred strains of mice (2003)

Crabbe JC, Metten P, Yu CH, Schlumbohm JP, Cameron AJ, Wahlsten D. Genotypic Differences in Ethanol Sensitivity in Two Tests of Motor Incoordination. J Appl Physiol. 2003 Oct;95(4):1338-51. Epub 2003 Apr 18.   PubMed 12704090  


         
Notes
The investigator selected the most useful measurements for MPD accessioning. Supplemental data set includes scores for hanging or falling from the balance beam, raw misstep data, and grid data from 5-min intervals.

Crabbe1 downloads
• Download Crabbe1 project data set     one row per animal, one column per trait
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• Crabbe1 supplementary data
Participants John C Crabbe       Oregon Health & Science University Portland, OR
Douglas Wahlsten       University of Windsor Windsor, Ontario CANADA
With: Metten P, Yu C-H, Schlumbohm JP, Cameron AJ
ContactJohn C Crabbe     crabbe@ohsu.edu
AcknowledgementsFunding provided by NIH AA10760, AA12714; Department of Veterans Affairs; Natural Sciences and Engineering Research Council of Canada (NSERC 45825).

Acknowledgements: Technical assistance provided by Best KL, Cotnam CJ, Dorow JA, Sibert JR, Zuraw JM; informatics assistance provided by Bobo M.
Project type Phenotype strain survey data set
MPD identifiersCrabbe1     MPD:154
Data changelog 1 update/correction.       Initial release date: 11/2003.
Formatted citation
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An investigation of the genetic determinants of ethanol sensitivity and intoxication. Animals were assayed for motor and/or behavioral incoordination following saline administration in control vs. ethanol treated mice using balance beam and grid test apparatus. Four i.p. treatment group cohorts: (1) saline; (2) ethanol 1.0 mg/kg body weight; (3) ethanol 1.2 mg/kg; (4) ethanol 1.4 mg/kg.

Summary of procedures that were conducted
• balance beam Ethanol i.p. (several doses) vs. control (saline). Missteps count. 3 beam lengths.
• grid test Ethanol injection (several doses) vs. control (saline). Grid habituation, missteps rate.
• body weight
Mice: inbred   8 strains   ♀♂   age 8wks   4 cohorts

Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Crabbe1 15402 body weight (none) body weight   [g]  bw inbred 8 both 8-16wks N=34
    Crabbe1 15403 balance beam ethanol missteps crossing balance beam   [n]     12.7mm beam:    control    i.p. doses g/kg:    1.0    1.2    1.4 inbred 7 both 8-16wks N=4
    Crabbe1 15404 balance beam ethanol missteps crossing balance beam   [n]     15.8mm beam:    control    i.p. doses g/kg:    1.0    1.2    1.4 inbred 8 both 8-16wks N=5
    Crabbe1 15405 balance beam ethanol missteps crossing balance beam   [n]     19.0mm beam:    control    i.p. doses g/kg:    1.0    1.2    1.4 inbred 8 both 8-16wks N=4
    Crabbe1 15406 grid test ethanol grid habituation, distance traveled   [cm]     control    i.p. doses g/kg:    1.5    2.0    2.5 inbred 8 both 8-16wks N=5
    Crabbe1 15407 grid test ethanol grid habituation, missteps per cm   [n/cm]     control    i.p. doses g/kg:    1.5    2.0    2.5 inbred 8 both 8-16wks N=5
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