Chesler3: Multi-system assessment of 8 inbred founder strains and 360+ emerging lines (pre-CC) of the Collaborative Cross (2011)

Philip VM, Sokoloff G, Ackert-Bicknell CL, Striz M, Branstetter L, Beckmann MA, Spence JS, Jackson BL, Galloway LD, Barker P, Wymore AM, Hunsicker PR, Durtschi DC, Chesler EJ, et al. Genetic analysis in the Collaborative Cross breeding population. Genome Res. 2011 Aug;21(8):1223-38. Epub 2011 Jul 6.   PubMed 21734011     FullText


       
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Participants Elissa J Chesler       The Jackson Laboratory Bar Harbor, ME
Vivek M Philip       The Jackson Laboratory Bar Harbor, ME
Brynn H Voy       University of Tennessee Knoxville, TN
With: Sokoloff G, Ackert-Bicknell C, Striz M, Churchill GA, Lariviere WR, Palmer AA, OHara BF
ContactElissa J Chesler     elissa.chesler@jax.org     Lab web site
Part of a series: • F1s with progenitors   see Chesler2
• pre-CC lines w/ progenitors   (this project) Chesler3
AcknowledgementsFunding provided by NIH DA021198, MH079103, AG025707; FA9550-05-1-0464; US Department of Energy, University of Tennessee Health Science Center, Oak Ridge National Laboratory, The Jackson Laboratory, The Ellison Medical Foundation
Project type Phenotype strain survey data set
MPD identifiersChesler3     MPD:435
Data changelog 2 updates/corrections.       Initial release date: 12/2012.
Formatted citation
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An assessment of emerging CC lines. Generations range from G2:F5 to G2:F9. Data for the 8 founder strains are also present.

Summary of procedures that were conducted
• metabolic panel Clinical blood chemistry. 16h fast.
• complete blood count Hematology.
• colony observation Reproduction, fecundity.
• open field test Locomotor activity, exploratory, anxiety-related behavior. 10 min test.
• light-dark box Anxiety, avoidance behavior. 10 min test.
• behavior observation Wildness scoring.
• nociception assay Hot plate and pressure on tail.
• monitoring system Sleep patterns. Dark period, total, peak activity. Baseline and after sleep deprivation.
• body size and weight Body weight, tail length.
• fat pad weights Gonadal fat pad weights.
• bone dimensions Femur.
• organ weights Heart, brain, kidney, spleen, gonadal fat pads, testicle weights.
• organ dimensions Kidney length and volume.
Mice: CC pre   367 strains including progenitors   ♀♂   age 7-15wks   1 cohort

Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Chesler3 43501 metabolic panel (none) sodium (blood Na)   [mmol/L]  sodium CC pre w/CC7 155 both 7-15wks N=1
    Chesler3 43502 metabolic panel (none) potassium (blood K)   [mmol/L]  potassium CC pre w/CC7 154 both 7-15wks N=1
    Chesler3 43503 metabolic panel (none) chloride (blood Cl)   [mmol/L]  chloride CC pre w/CC7 153 both 7-15wks N=1
    Chesler3 43504 metabolic panel (none) calcium (blood ionized Ca)   [mmol/L]  calcium CC pre w/CC7 154 both 7-15wks N=1
    Chesler3 43505 metabolic panel (none) anion gap concentration (mEq/L)   [mmol/L]  anion_gap CC pre w/CC7 148 both 7-15wks N=1
    Chesler3 43506 metabolic panel (none) carbon dioxide (blood CO2)   [mmol/L]  carbon_dioxide CC pre w/CC7 154 both 7-15wks N=1
    Chesler3 43507 metabolic panel (none) glucose (blood GLU, no fast)   [mg/dL]  GLU CC pre w/CC7 155 both 7-15wks N=1
    Chesler3 43508 metabolic panel (none) glucose (blood GLU, 16h fast)   [mg/dL]  GLU_fast CC pre w/CC8 257 both 7-15wks N=2
    Chesler3 43509 metabolic panel (none) blood urea nitrogen (BUN)   [mg/dL]  BUN CC pre w/CC7 152 both 7-15wks N=1
    Chesler3 43510 metabolic panel (none) creatinine (blood)   [mg/dL]  creatinine CC pre w/CC7 154 both 7-15wks N=1
    Chesler3 43513 complete blood count (none) white blood cell count (WBC; units per volume x 103)   [n/µL]  WBC CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43514 complete blood count (none) lymphocyte count (LYM; units per volume x 103)   [n/µL]  LYM CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43515 complete blood count (none) lymphocyte differential (LYM; percent of viable WBC)   [%]  pct_LYM CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43516 complete blood count (none) monocyte count (MONO; units per volume x 103)   [n/µL]  MONO CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43517 complete blood count (none) monocyte differential (MONO; percent of viable WBC)   [%]  pct_MONO CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43518 complete blood count (none) granulocyte count (GRA; units per volume x 103)   [n/µL]  GRA CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43519 complete blood count (none) granulocyte differential (GRA; percent of total viable WBC)   [%]  pct_GRA CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43520 complete blood count (none) eosinophil differential (EOS; percent of viable WBC)   [%]  pct_EOS CC pre w/CC8 152 both 7-15wks N=1
    Chesler3 43521 complete blood count (none) red blood cell count (RBC; units per volume x 106)   [n/µL]  RBC CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43522 complete blood count (none) mean RBC corpuscular volume (MCV)   [fL]  MCV CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43523 complete blood count (none) RBC corpuscular distribution width (RDW)   [%]  RDW CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43524 complete blood count (none) mean RBC corpuscular hemoglobin (MCH)   [pg]  MCH CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43525 complete blood count (none) mean RBC corpuscular hemoglobin concentration (MCHC)   [g/dL]  MCHC CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43526 complete blood count (none) hemoglobin (HGB)   [g/dL]  hemoglobin CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43527 complete blood count (none) hematocrit (HCT)   [%]  hematocrit CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43528 complete blood count (none) platelet count (PLT; units per volume x 103)   [n/µL]  platelets CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43529 complete blood count (none) mean platelet volume (MPV)   [fL]  MPV CC pre w/CC8 154 both 7-15wks N=1
    Chesler3 43540 organ weights (none) testicular weight   [g]  testes_wt CC pre w/par 54 both 4wks N=1
    Chesler3 43541 colony observation (none) number of mice born in first litter   [n]  litter_size CC pre w/CC8 299 both 4wks N=2
    Chesler3 43542 colony observation (none) parental age at first litter   [d]  age_first_litter CC pre w/CC8 299 both 4wks N=2
    Chesler3 43543 colony observation (none) time from mate to first litter   [d]  duration_to_litter CC pre w/CC8 299 both 4wks N=2
    Chesler3 43551 open field test (none) distance traveled during first 3 min, 10 min test   [cm]  distance3_OFT CC pre w/par 198 both 7-15wks N=1
    Chesler3 43552 open field test (none) distance traveled, 10 min test   [cm]  distance10_OFT CC pre w/par 198 both 7-15wks N=1
    Chesler3 43553 open field test (none) number of transitions between center and periphery, 10 min test   [n]  transitions_OFT CC pre w/par 197 both 7-15wks N=1
    Chesler3 43554 open field test (none) time spent in center during first minute, 10 min test   [s]  center1_OFT CC pre w/par 198 both 7-15wks N=1
    Chesler3 43555 open field test (none) time spent in center, 10 min test   [s]  center10_OFT CC pre w/par 198 both 7-15wks N=1
    Chesler3 43556 open field test (none) integrated distance from absolute center, 10 min test   [cm]  distance_center_OFT CC pre w/par 196 both 7-15wks N=1
    Chesler3 43557 open field test (none) percentage of time in center, 10 min test   [%]  pct_center_OFT CC pre w/par 198 both 7-15wks N=1
    Chesler3 43558 open field test (none) latency to reach periphery, 10 min test   [s]  latency_periphery_OFT CC pre w/par 197 both 7-15wks N=1
    Chesler3 43559 open field test (none) time spent in periphery, 10 min test   [s]  duration_periphery_OFT CC pre w/par 198 both 7-15wks N=1
    Chesler3 43560 open field test (none) time spent immobile during first minute, 10 min test   [s]  immobile_OFT CC pre w/par 196 both 7-15wks N=1
    Chesler3 43561 open field test (none) number of fecal boli, 10 min test   [n]  fecal_boli_OFT CC pre w/par 184 both 7-15wks N=1
    Chesler3 43562 open field test (none) number of urine puddles, 10 min test   [n]  urination_OFT CC pre w/par 184 both 7-15wks N=1
    Chesler3 43563 light-dark box (none) time spent in light compartment, 10 min test   [s]  duration_light_LD CC pre w/par 152 both 7-15wks N=1
    Chesler3 43564 light-dark box (none) distance traveled in light compartment, 10 min test   [cm]  distance_light_LD CC pre w/par 152 both 7-15wks N=1
    Chesler3 43565 light-dark box (none) percentage of time in light compartment, 10 min test   [%]  pct_light_LD CC pre w/par 154 both 7-15wks N=1
    Chesler3 43566 light-dark box (none) latency to enter dark compartment, 10 min test   [s]  latency_dark_LD CC pre w/par 156 both 7-15wks N=1
    Chesler3 43567 light-dark box (none) number of transitions between light and dark compartments, 10 min test   [n]  transitions_LD CC pre w/par 154 both 7-15wks N=1
    Chesler3 43568 light-dark box (none) number of fecal boli, 10 min test   [n]  fecal_boli_LD CC pre w/par 150 both 7-15wks N=1
    Chesler3 43569 light-dark box (none) number of urine puddles, 10 min test   [n]  urination_LD CC pre w/par 149 both 7-15wks N=1
    Chesler3 43570 behavior observation (none) response to capture   [score]  capture_score CC pre w/CC8 324 both 7-15wks N=2
    Chesler3 43571 behavior observation (none) response to holding   [score]  hold_score CC pre w/CC8 324 both 7-15wks N=2
    Chesler3 43572 behavior observation (none) capture and hold scores combined   [score]  wildness_score CC pre w/CC8 324 both 7-15wks N=2
    Chesler3 43573 nociception assay (none) latency of nociceptive response to pressure on the tail, 60 s test   [s]  tail_clip CC pre w/CC7 294 both 7-15wks N=2
    Chesler3 43574 nociception assay (none) latency of nociceptive response to hot plate (54°C), 30 s test   [s]  hot_plate CC pre w/CC7 292 both 7-15wks N=2
    Chesler3 43581 monitoring system (none) percent of time sleeping, baseline   [%]     total    dark period CC pre w/CC8 260 both 7-15wks N=2
    Chesler3 43583 monitoring system (none) percent of time sleeping, after sleep deprivation   [%]     total    dark period    peak activity CC pre w/CC8 255 both 7-15wks N=2
    Chesler3 43586 body size and weight (none) body weight   [g]  body_weight CC pre w/CC8 335 both 7-15wks N=3
    Chesler3 43587 body size and weight (none) body length   [cm]  body_length CC pre w/par 252 both 7-15wks N=2
    Chesler3 43588 body size and weight (none) tail length   [cm]  tail_length CC pre w/CC8 324 both 7-15wks N=2
    Chesler3 43589 fat pad weights (none) gonadal fat pad weight   [g]  gonad_fat_wt CC pre w/CC8 321 both 7-15wks N=2
    Chesler3 43590 bone dimensions (none) periosteal (outer) circumference, femoral mid-diaphysis   [mm]  bone_circumference CC pre w/par 199 both 7-15wks N=1
    Chesler3 43591 organ weights (none) brain weight   [g]  brain_wt CC pre w/par 149 both 7-15wks N=1
    Chesler3 43592 organ weights (none) heart weight   [g]  heart_wt CC pre w/CC8 324 both 7-15wks N=2
    Chesler3 43593 organ dimensions (none) kidney length   [cm]     left    right CC pre w/par 113 both 7-15wks N=1
    Chesler3 43595 organ dimensions (none) kidney volume   [cm3]  kidney_vol CC pre w/par 69 both 7-15wks N=1
    Chesler3 43596 organ weights (none) kidney weight   [g]     left    right    both CC pre w/CC8 323 both 7-15wks N=2
    Chesler3 43599 organ weights (none) spleen weight   [g]  spleen_wt CC pre w/par 228 both 7-15wks N=2
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