Brockmann1: Genetic factors of body, muscle, fat weight and their pleiotropic effects in F2 progeny from 2 intercross populations (2009)

Brockmann GA, Tsaih SW, Neuschl C, Churchill GA, Li R. Genetic factors contributing to obesity and body weight can act through mechanisms affecting muscle weight, fat weight, or both. Physiol Genomics. 2009 Jan 8;36(2):114-26. Epub 2008 Nov 4.   PubMed 18984673     FullText


     
Brockmann1 downloads
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Use the QTL Data Downloads button near the bottom of this page.
Participants G A Brockmann
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Tsaih SW, Neuschl C, Li R
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsThe project was supported from the Federal Ministry for Education and Research, Germany (NGFN project 01GS0486, G. A. Brockmann),Deutsche Forschungsgemeinschaft (GRK 1208, C. Neuschl), and National Institute of General Medical Sciences (GM-076468, G. A. Churchill).
Project type QTL Archive data set
MPD identifiersBrockmann1     MPD:213
Data changelog No updates/corrections.       Initial release date: 09/2010.
This project is to investigate the relationships between genetic loci and different phenotypes influencing body weight. Two F2 populations were generated: Cross 1 is an intercross of DBA/2 and NMRI8; Cross 2 is an intercross of DBA/2 and DU6i. For both crosses the F2 progeny were fed breeding diet (1) and phenotyped for body weight, muscle weight, fat weight and other related phenotypes (studied phenotypes vary in cross1 vs. cross2). For detailed protocols see Brockmann et al 2009.

(1) Breeding diet: containing 12.5 MJ/kg metabolic energy with an average content of 22.5% crude protein, 5.0% crude fat, 4.5% crude fiber, 6.5% crude ash, 13.5% water, 48.0% N-free extract, vitamins, trace elements, amino acids, and minerals (Altromin diet 1314).


QTL data set parameters:
Cross 1: DBA/2 x NMRI8       Brockmann1
Progenitor strains DBA/2J     NMRI8    
Cross direction notation(DBA/2 x NMRI8) F1 x (DBA/2 x NMRI8) F1
Cross typeF2 intercross
Population size 142 ♀     133 ♂

Cross 2: DBA/2 x DU6i       Brockmann1b
Progenitor strains DBA/2J     DU6i    
Cross direction notation(DBA/2 x DU6i) F1 x (DBA/2 x DU6i) F1
Cross typeF2 intercross
Population size 174 ♀     228 ♂


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
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Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Brockmann1 21301 colony observation (none) number of offspring per litter: 3 classes   [n]  pupsize QTL population both 6wks N=138
Brockmann1 21302 body weight (none) mouse body weight at week 3   [g]  Bw3 QTL population both 3wks N=138
Brockmann1 21303 body weight (none) mouse body weight at week 4   [g]  Bw4 QTL population both 4wks N=138
Brockmann1 21304 body weight (none) mouse body weight at week 5   [g]  Bw5 QTL population both 5wks N=138
Brockmann1 21305 body weight (none) mouse body weight at week 6   [g]  Bw6 QTL population both 6wks N=138
Brockmann1 21306 organ weights (none) abdominal fat   [mg]  Afw QTL population both 6wks N=138
Brockmann1 21307 organ weights (none) abdominal fat percentage = %(Afw/body weight)   [%]  Afp QTL population both 6wks N=138
Brockmann1 21308 muscle weights (none) muscle weight   [mg]  Mw QTL population both 6wks N=138
Brockmann1 21309 organ weights (none) weight of liver   [mg]  Liver QTL population both 6wks N=138
Brockmann1 21310 organ weights (none) weight of kidney   [mg]  Kidney QTL population both 6wks N=138
Brockmann1 21311 organ weights (none) weight of spleen   [mg]  Spleen QTL population both 6wks N=138
Brockmann1 21501 colony observation (none) number of offspring per litter: 5 classes   [n]  pupsize QTL population both 6wks N=201
Brockmann1 21502 body weight (none) mouse body weight at week 6   [g]  bw6 QTL population both 6wks N=201
Brockmann1 21503 organ weights (none) abdominal fat weight   [mg]  afw QTL population both 6wks N=201
Brockmann1 21504 organ weights (none) abdominal fat percentage = %(Afw/body weight)   [%]  afp QTL population both 6wks N=201
Brockmann1 21505 muscle weights (none) muscle weight   [mg]  mw QTL population both 6wks N=201
Brockmann1 21506 organ weights (none) weight of liver   [mg]  liver QTL population both 6wks N=201
Brockmann1 21507 organ weights (none) weight of kidney   [mg]  kidney QTL population both 6wks N=201
Brockmann1 21508 organ weights (none) weight of spleen   [mg]  spleen QTL population both 6wks N=201
Brockmann1 21509 hormone quantification (none) leptin level   [ng/mL]  leptin QTL population both 6wks N=201
Brockmann1 21510 hormone quantification (none) insulin level   [pmol/L]  insulin QTL population both 6wks N=201
Brockmann1 21511 hormone quantification (none) IGF-1 level   [ng/mL]  igf1 QTL population both 6wks N=201
Brockmann1 21512 hormone quantification (none) leptin level, log10 transformed   [log(ng/mL)]  lg_lept QTL population both 6wks N=201
Brockmann1 21513 hormone quantification (none) insulin level, log10 transformed   [log(pmol/L)]  lg_ins QTL population both 6wks N=201
Brockmann1 21514 hormone quantification (none) IGF1 level, log10 transformed   [log(ng/mL)]  lg_igf1 QTL population both 6wks N=201
Brockmann1 21515 lipid profile (none) triglyceride  tg QTL population both 6wks N=201
Brockmann1 21516 lipid profile (none) total cholesterol  chol QTL population both 6wks N=201
Brockmann1 21517 lipid profile (none) HDL cholesterol  hdl QTL population both 6wks N=201
Brockmann1 21518 lipid profile (none) triglycerides, log transformed  lg_tg QTL population both 6wks N=201
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Cross 1: DBA/2 x NMRI8     Brockmann1     a.k.a. Brockmann_2009a
Excel file:   Data_Description_DBA2xNMRI8_Brockmann2009.xlsx
CSV file:   Brockmann2009_DBA2xNMRI8_B37_Data.csv
Readme / text file:   readme_Brockmann2009a.txt
QC plots, figures, etc.:   QCReport_Brockmann2009a.pdf

Cross 2: DBA/2 x DU6i     Brockmann1b     a.k.a. Brockmann_2009b
Excel file:   Data_Description_DBA2xDU6i_Brockmann2009.xlsx
CSV file:   Brockmann2009_DBA2xDU6i_B37_Data.csv
Readme / text file:   readme_Brockmann2009b.txt
QC plots, figures, etc.:   QCReport_Brockmann2009b.pdf