MPD: Data set: Wittenburg1

Wittenburg1: Cholesterol gallstones, gallbladder mucin accumulation, and related gallstone phenotypes in reciprocal backcrosses between AKR/J and SWR/J (2002)

Wittenburg H, Lammert F, Wang DQ, Churchill GA, Li R, Bouchard G, Carey MC, Paigen B. Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice. Physiol Genomics. 2002 Feb 11;8(1):67-77. PubMed 11842132

Wittenburg1 downloads
QTL projects have several available downloads.
Use the QTL Data Downloads button near the bottom of this page.

Ontology terms mapped to Wittenburg1 measures:

MA:0000356 gall bladder
MA:0000358 liver
MA:0002513 bile
MP:0001259 abnormal body weight
MP:0004773 abnormal bile composition
MP:0004847 abnormal liver weight
MP:0020131 abnormal gallbladder volume
VT:0001259 body mass
VT:0003402 liver mass
VT:0005084 gall bladder morphology trait
VT:0005675 gall bladder size trait

Investigators	H Wittenberg Gary A Churchill The Jackson Laboratory, Bar Harbor, ME
Participants	Lammert F, Wang DQ, Li R, Bouchard G, Carey MC, Paigen B
Contact	Gary A Churchill gary.churchill@jax.org
Affiliated Center	QTL Archive
Acknowledgements	Grant: This work was supported in part by National Institute of Diabetes and Digestive and Kidney Diseases Grants DK-51568 (to B. Paigen), DK-36588, DK-52911, and DK-34854 (to M. C. Carey), DK-54012 (to D. Q.-H. Wang), and by a grant from the Ellison Medical Foundation (to D. Q.-H. Wang). F. Lammert (La 997/3-1) and H. Wittenburg (WI 1905/1-1) were supported by the Deutsche Forschungsgemeinschaft.
Project type	QTL Archive data set
MPD identifiers	Wittenburg1 MPD:126
Data changelog	No updates/corrections. Initial release date: 01/2008.

A reciprocal backcross of AKR/J and SWR/J was generated to identify QTLs for cholesterol gallstones, gallbladder mucin accumulation, liver and body weight. At 8 wks of age, backcross progeny of both sexes were administered a high-fat (15%) diet for 12 wks and examined for gallstone formation and related phenotypes. Mice were fasted prior to sample collection. For detailed protocols see Wittenburg et al 2002.

QTL data set parameters:

Progenitor strains	AKR/J SWR/J
Cross direction notation	(SWR x AKR) x AKR; AKR x (SWR x AKR)
Cross type	Reciprocal backcross
Population size	41 ♀ 220 ♂
Age	18-20wks

Measured phenotypes: To access these data use the QTL Data Downloads button at bottom of page.

Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Wittenburg1	body weight	high-fat diet	body weight, log transformed [log(g)] bw_log 12601	QTL population	both	18-20wks	N=130

Wittenburg1	organ weights	high-fat diet	liver weight [g] liver_wt 12602	QTL population	both	18-20wks	N=130

Wittenburg1	organ weights	high-fat diet	liver-to-bw ratio [ratio] liver_bw_ratio 12603	QTL population	both	18-20wks	N=130

Wittenburg1	organ dimensions	high-fat diet	gallbladder, volume [µL] gallbladder_vol 12604	QTL population	both	18-20wks	N=130

Wittenburg1	histopathology	high-fat diet	gallbladder, mucin (score 0-4), log transformed [score] gallbladder_mucin_log 12605	QTL population	both	18-20wks	N=130

Wittenburg1	histopathology	high-fat diet	gallstones, presence (score 0=absent, 1=present) [score] gallstone_presence 12606	QTL population	both	18-20wks	N=130

Wittenburg1	histopathology	high-fat diet	gallstones, number [n] gallstone_count 12607	QTL population	both	18-20wks	N=130

Wittenburg1	histopathology	high-fat diet	weight of solid gallstones [mg] gallstone_wt 12608	QTL population	both	18-20wks	N=130

Wittenburg1	histopathology	high-fat diet	gallstone severity (score 0=absent, 1=crystals, 2=stones) [score] gallstone_score 12609	QTL population	both	18-20wks	N=130

Wittenburg1	histopathology	high-fat diet	gallstones volume gallstone_vol 12610	QTL population	both	18-20wks	N=130

Associated genomic features

MGI-curated QTL / genomic features that have been associated with this data set:
Lith1 Lith2 Lith3 Lith4 Lith5

Project / data set: Wittenburg1 a.k.a. Wittenburg_2002
Excel file: Data_Description_SWRxAKR_BC_Wittenburg2002.xlsx
CSV file: Wittenburg2002_SWRxAKR_BC_B37_Data.csv
Readme / text file: readme_Wittenburg2002.txt