Bennett3_Animal
Acclimation
and
testing
periods
Supplier |
All
breeding
and
testing
of
live
mice*
were
conducted
in
the
Specific
Pathogen
Free
(SPF)
facility
at
the
Institute
for
Behavioral
Genetics,
University
of
Colorado
at
Boulder. |
| Acclimation |
1-2
wks
before
testing |
| Ages |
8-14
wks
of
age
at
time
of
testing |
Feed |
Sterilized
rodent
diet
by
Teklad
(4%
fat,
24%
protein)
given
ad
libitum |
Water |
Given
ad
libitum
|
Housing |
Mice
of
the
same
strain
and
sex
were
housed
together
at
weaning,
~21
to
25
days
of
age;
no
singly
housed
mice
were
tested |
Bedding |
Harlan
Softcob
bedding |
Photoperiod |
12:12
hr
L:D;
lights
on
at
0700;
lit
with
fluorescent
ceiling
lights |
| Humidity |
12%
relative |
Temperature |
22
±
1
°C |
Regulatory
compliance |
All
procedures
were
approved
by
the
University
of
Colorado
Institutional
Animal
Care
and
Use
Committee,
in
accordance
with
National
Institute
of
Health
guidelines. |
*The
ILSXISS#/Tej
(formerly
LXS)
recombinant
inbred
lines
were
generated
from
crosses
between
ILS/Ibg
(Inbred
Long
Sleep)
and
ISS/Ibg
(Inbred
Short
Sleep).
These
two
progenitor
strains
were
developed
from
the
LS
and
SS
selected
lines
developed
from
a
heterogenous
stock
derived
from
an
8
way
cross
(A,
AKR,
BALB/c,
C3H/2,
C57BL,
DBA/2,
IS/Bi
and
RIII).
ILS
and
ISS
are
the
product
of
inbreeding
LS
and
SS,
lines
selected
for
long
or
short
duration
of
loss-of-righting
response
following
high-dose
intraperitoneal
administration
of
ethanol.
To
produce
RI
strains,
ILS
and
ISS
mice
were
intercrossed
and
4
reciprocal
matings
of
F1
mice
were
used
to
produce
the
F2
generation.
Each
of
the
four
F2
crosses
generated
a
unique
combination
of
Y
Chromosome
and
maternal
(mitochondrial)
contributions.
Breedings
were
conducted
in
the
Specific
Pathogen
Free
(SPF)
facility
at
the
Institute
for
Behavioral
Genetics,
University
of
Colorado
at
Boulder
(see
Bennett
et.
al.,
2005).
|
|
