MPD: Data set: Schughart5

Leist SR, Pilzner C, van den Brand JM, Dengler L, Geffers R, Kuiken T, Balling R, Kollmus H, Schughart K. Influenza H3N2 infection of the collaborative cross founder strains reveals highly divergent host responses and identifies a unique phenotype in CAST/EiJ mice. BMC Genomics. 2016 Feb 27;17:143. doi: 10.1186/s12864-016-2483-y. PubMed 26921172 FullText

Schughart5 downloads
• Download Schughart5 project data set animal data, as uploaded
• Download Schughart5 animal data matrix with factor-related expansion applied as necessary
• Download Schughart5 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Schughart5 measures:

MP:0001259 abnormal body weight
MP:0005629 abnormal lung weight
MP:0020185 altered susceptibility to viral infection
VT:0001259 body mass
VT:0005629 lung mass
VT:0010439 response to viral infection trait

Investigators	Sarah R Leist Helmholtz Center for Infection Research, Braunschweig GERMANY Heike Kollmus Helmholtz Center for Infection Research, Braunschweig GERMANY Klaus Schughart Helmholtz Center for Infection Research, Braunschweig GERMANY
Contact	Klaus Schughart Klaus.Schughart@helmholtz-hzi.de Lab web site
Part of a series:	• study 1 see Schughart3 • study 2 see Schughart4 • study 3 (this project) Schughart5
Project type	Phenotype strain survey data set
MPD identifiers	Schughart5 MPD:588
Data changelog	No updates/corrections. Initial release date: 01/2017.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice infected with influenza A H3N2 virus (one dose) were analyzed for daily weight loss for up to 30 days. The mouse-adapted virus strain influenza A/HK/01/68 was used. Lung weights are also available. Overall survival and mortality rates are available from females and males in Schughart3, and viral loads and leukocyte profiles are available in Schughart4.

Procedures conducted:

• body weight		Influenza A (H3N2) virus vs. controls. Several timepoints.
• organ weights		Lung weight. Animals infected with influenza A (H3N2) virus vs. controls.

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Schughart5:1	Schughart5	body weight	(none)	body weight pre-infection [g] bw 58801 control group treated group	inbred w/CC8	8	f	8-12wks	N=8

Schughart5:11	Schughart5	body weight	(none)	body weight as percent of baseline [%] pctbw 58811 days post-treatment (saline): 1 2 3	inbred w/CC8	8	f	8-12wks	N=4

Schughart5:21	Schughart5	body weight	influenza A (H3N2) virus	body weight as percent of baseline, 10 FFU group [%] pctbw_trt 58821 days post-infection: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 18 30	inbred w/CC8	8	f	8-12wks	N=9

Schughart5:81	Schughart5	organ weights	influenza A (H3N2) virus	lung weight, control group, day 3 [g] lungwt_ctrl_d3 58881	inbred w/CC8	8	f	10wks	N=3

Schughart5:83	Schughart5	organ weights	influenza A (H3N2) virus	lung weight, treated group [g] lungwt_trt 58883 days post-infection: 3 5 8 18 30	inbred w/CC8	8	f	10wks	N=4

Schughart5:91	Schughart5	organ weights	influenza A (H3N2) virus	relative lung weight, control group, day 3 [%] lungwtadj_ctrl_d3 58891	inbred w/CC8	8	f	10wks	N=3

Schughart5:93	Schughart5	organ weights	influenza A (H3N2) virus	relative lung weight, treated group [%] lungwtadj_trt 58893 days post-infection: 3 5 8 18 30	inbred w/CC8	8	f	10wks	N=4

Schughart5: Susceptibility to influenza A (H3N2) viral infection: body weight and lung weight in females of the 8 inbred founder strains of the Collaborative Cross (2016)