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Project data set: Schonfeld1   (2005)

Project Strain survey of liver and plasma lipids and blood glucose levels in 11 inbred strains of mice

An investigation of liver triglyceride (TG) levels and development of fatty liver following fasting. Animals were surveyed for blood glucose/insulin metabolism and tolerance, blood and liver lipid metabolism, liver and body weights, and overall fat composition.

Study design: baseline survey; 1 cohort.
11 strains tested     female male     Test age: 9-13wks

Investigators Schonfeld G   [Details, papers]

Protocol View phenotyping protocol and methods

Schonfeld1
measurements



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blood — clinical chemistry         metabolic panel  
Schonfeld1
glucose (plasma GLU, non-fasted)   [mg/dL]
10 strains   age 9-13wks
  Inbred
 
blood — clinical chemistry         glucose tolerance  
Schonfeld1
glucose levels during glucose tolerance test
(10% glucose i.p., 5h fast) [mg/dL]  
      • 0min  • 30min  • 60min  • 120min    • time comparison  
10 strains   age 9-13wks
  Inbred
Schonfeld1
glucose levels, area under curve, glucose tolerance test
(10% glucose i.p., 5h fast) [AUC]  
      • 0 to 60min  • 0 to 120min    • time comparison  
10 strains   age 9-13wks
  Inbred
 
blood — clinical chemistry         insulin tolerance  
Schonfeld1
glucose levels as percent of baseline, insulin tolerance test
(human insulin 0.75 U/kg bw i.p., 5h fast) [%]  
      • 0min  • 30min  • 60min  • 120min    • time comparison  
10 strains   age 9-13wks
  Inbred
Schonfeld1
glucose levels, area under curve, insulin tolerance test
(human insulin 0.75 U/kg bw i.p., 5h fast) [AUC]  
      • 0 to 60min  • 0 to 120min    • time comparison  
10 strains   age 9-13wks
  Inbred
 
blood — clinical chemistry         metabolic panel  
Schonfeld1
beta-hydroxybutyrate (plasma BHB, 5h fast)   [mM]
10 strains   age 9-13wks
  Inbred
 
blood — lipids         lipid profile  
Schonfeld1
free cholesterol (plasma, 5h fast)   [mg/dL]
10 strains   age 9-13wks
  Inbred
Schonfeld1
cholesterol ester (plasma, 5h fast)   [mg/dL]
10 strains   age 9-13wks
  Inbred
Schonfeld1
total cholesterol (plasma CHOL, 5h fast)   [mg/dL]
10 strains   age 9-13wks
  Inbred
Schonfeld1
free fatty acids (plasma FFA, 5h fast)   [mol/m3]
10 strains   age 9-13wks
  Inbred
Schonfeld1
phospholipids (plasma, 5h fast)   [mg/dL]
10 strains   age 9-13wks
  Inbred
Schonfeld1
triglycerides (plasma TG, 5h fast)   [mg/dL]
10 strains   age 9-13wks
  Inbred
 
body composition         DXA  
Schonfeld1
percent fat, head excluded   [%]
10 strains   age 9-13wks
  Inbred
 
body weight         scales  
Schonfeld1
body weight   [g]
10 strains   age 9-13wks
  Inbred
 
endocrine — hormones         radioimmunoassay  
Schonfeld1
insulin level (plasma), 30 min post injection in glucose tolerance test
(10% glucose i.p., 5h fast)
  [ng/mL]
10 strains   age 9-13wks
  Inbred
 
liver — lipid homeostasis         lipid profile  
Schonfeld1
hepatic triglycerides (5h fast)   [mg/g]
10 strains   age 9-13wks
  Inbred
 
liver — weight         scales  
Schonfeld1
liver weight   [g]
10 strains   age 9-13wks
  Inbred
Schonfeld1
liver weight as a percent of body weight   [%]
10 strains   age 9-13wks
  Inbred


local experiment parameter  
Schonfeld1
age at testing   [wks]
10 strains  
  Inbred



Number of mice tested per strain, for each measurement

  
Changelog There has been one update or correction to the data set since MPD release in Dec 2009  
Reference
Lin X, Yue P, Chen Z, Schonfeld G. Hepatic triglyceride contents are genetically determined in mice: results of a strain survey. Am J Physiol Gastrointest Liver Physiol. 2005 Jun;288(6):G1179-89. Epub 2004 Dec 9.     PubMed 15591160     MGI

Related papers
Mice
Strain and animal info
Note
An analysis (3 strains) of the effect of different fasting times (0h, 6h, 14h) for BHB, FFA, and hTG is available here.
Note
Please note: Low sample sizes (N < 5) are present. [View table]
Note
Some measurements are a mixture of + and – values. [View list]
Note
Some measurements are not eligible for all-MPD pheno vs. pheno
correlations due to data characteristics or semantics [View list]

Schonfeld1
downloads


Individual animal data
Table format (.csv)
Individual animal data in table format, with one row per animal and one column per measurement.
Individual animal data
List format (.csv)
Individual animal data organized as one row per animal / measurement reading.
Strain means (.csv) Strain means with SD, N, other summary statistics. One row per strain / sex / measurement.
Supplemental data are available

 See also the MPD download center for more general data download options.





 




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