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Data set: Berndt2   (2010)

  Unrestrained plethysmograph and anesthetized forced oscillation methods of measuring lung function in 29 inbred strains of mice
  Mice were assessed for respiratory parameters at baseline and then again after methacholine (MCh) challenge using two different techniques in two cohorts: 1) unrestrained plethysmograph technique (BUXCO), given MCh doses of 5, 10, and 20 mg/mL; 2) anesthetized forced oscillation technique (FlexiVent), given MCh doses of 1, 3, 10, and 30 mg/mL . The "MCh response index" is the average slope of the MCh challenge series (see protocol for details).
 

Study design: baseline vs. intervention in various doses   2 cohorts.
29 strains tested (inbred)     female male     Test age: 9-12wks
Sample sizes are 8.2 on average. ... Caution, some N ≤ 2 are present  [details]

  Investigators: Berndt A, Leme AS, Paigen B, Shapiro SD, Svenson KL     [More info... papers, citing, funding]

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Berndt2
phenotype
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respiratory — breathing pattern — efficiency         plethysmography  
Berndt2
minute ventilation [mL/min]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
Berndt2
breath frequency [n/min]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
 
respiratory — breathing pattern — cycle         plethysmography  
Berndt2
inspiratory phase [s]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
Berndt2
expiratory phase [s]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
Berndt2
respiratory cycle [s]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
Berndt2
duty cycle [ratio]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
 
respiratory — breathing pattern — airflow rate         plethysmography  
Berndt2
inspiratory flow rate [mL/kg/s]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
Berndt2
expiratory flow rate [mL/kg/s]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
Berndt2
respiratory flow rate [mL/kg/s]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
 
respiratory — breathing pattern — enhanced pause         plethysmography  
Berndt2
enhanced pause     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
 
respiratory — lung volume         plethysmography  
Berndt2
tidal volume [mL]     baseline and  methacholine      
• saline  • 5 MCh  • 10 MCh  • 20 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
 
respiratory — airway resistance         ventilator  
Berndt2
airway resistance (H20 pressure) [cm/mL/s]     baseline and  methacholine      
• saline  • 1 MCh  • 3 MCh  • 10 MCh  • 30 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred
 
respiratory — lung compliance         ventilator  
Berndt2
lung tissue dynamic elastance (H20 pressure) [cm/mL]     baseline and  methacholine      
• saline  • 1 MCh  • 3 MCh  • 10 MCh  • 30 MCh  • response index      dose comparison  
29 strains   age 9-12wks
  Inbred




Some measurements are a mixture of + and – numeric values. [List]

One update or correction was posted since initial release in Mar 2011
 
Berndt A, Leme AS, Williams LK, Von Smith R, Savage HS, Stearns TM, Tsaih SW, Shapiro SD, Peters LL, Paigen B, Svenson KL. Comparison of unrestrained plethysmography and forced oscillation for identifying genetic variability of airway responsiveness in inbred mice. Physiol Genomics. 2011 Jan 7;43(1):1-1Epub 2010 Sep 7.     PubMed 20823217     MGI     FullText

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