blood — clinical chemistry — enzymes — ALT         metabolic panel   Paigen1 alanine aminotransferase (plasma ALT)   [IU/L] ...... 103   182 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Rusyn5 serum alanine aminotransferase (ALT) [IU/L]   high-fat diet, ethanol       • high-fat only  ...... 26.7 (N=3)         • high-fat and ethanol  ...... 60.4 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   blood — clinical chemistry — glucose         metabolic panel   Naggert1 glucose (plasma GLU, 4h fast) [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 190   229 ▲         • after 17wks on diet  ...... 175   153         • Δchange  ...... 0.967   0.647 ▼           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — clinical chemistry — miscellaneous         metabolic panel   Paigen1 bile salts (plasma)   [µmol/L] ...... 61.9   106 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred   blood — clinical chemistry — proteins — misc         HPLC   Rusyn5 homocysteine concentration (plasma) [nmol/mL]   high-fat diet, ethanol       • high-fat only  ...... 3.26 (N=3) ▼         • high-fat and ethanol  ...... 7.52 (N=2)           • comparison   14 strains   age 8-14wks   Inbred   blood — lipids — cholesterol — HDL         lipid profile   Paigen1 HDL cholesterol (plasma)   high-fat diet   8wks       • amount  ...... 85.8   121 (N=3)         • % of total plasma cholesterol  ...... 48.7   52.4 (N=3)         43 strains   age 15-19wks   Inbred Paigen2 HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 94.7 ▲   113 ▲         • after 17wks on diet  ...... 96.5   93.2         • Δchange  ...... 1.10   0.681 ▼           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — cholesterol — LDL or non-HDL         lipid profile   Paigen1 plasma cholesterol not in the HDL fraction   [mg/dL] ...... 91.0   117 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 non-HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 50.2 ▲   26.1         • after 17wks on diet  ...... 128   187         • Δchange  ...... 2.89   6.28 (N=4)           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — cholesterol — total         lipid profile   Paigen1 total cholesterol (plasma)   [mg/dL] ...... 177   239 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 total cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 145 ▲▲   139 ▲         • after 17wks on diet  ...... 225   281         • Δchange  ...... 1.55   1.81 (N=4)           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — triglycerides         lipid profile   Paigen2 triglycerides [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 198 ▲   142         • after 17wks on diet  ...... 100   82.3         • Δchange  ...... 0.482   0.517           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — xenobiotics — alcohol concentration         spectrophotometry   Rusyn5 blood alcohol concentration (BAC) [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 42.8 (N=3)         • high-fat and ethanol  ...... 343 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   body composition — fat — percent         DXA   Naggert1 percent of tissue mass that is fat, head excluded   [%] ...... 26.5   18.1     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — fat — weight         DXA   Naggert1 weight of fat portion of tissue mass, head excluded   [g] ...... 5.98   5.40     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — lean         DXA   Naggert1 weight of lean portion of tissue mass, head excluded   [g] ...... 16.5   24.3     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — total         DXA   Naggert1 total tissue mass, head excluded   [g] ...... 22.5   29.7     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body weight         scales   Naggert1 body weight   [g] ...... 23.3   30.7     high-fat diet   8wks 43 strains   age 15-17wks   Inbred Paigen1 body weight [g]     baseline and  high-fat diet 8wks       • start  ...... 20.1   25.4         • after 8wks on diet  ...... 24.2   28.6 (N=3)         • Δchange  ...... 1.21   1.16 (N=3)           • comparison   43 strains   age 7-11wks                 at baseline   Inbred   bone — mineral density and content — whole body without head         DXA   Naggert1 bone mineral density and content   high-fat diet   8wks       • BMD  ...... 0.0507   0.0527         • BMC  ...... 0.495   0.560         43 strains   age 15-17wks   Inbred   cardiovascular — atherosclerosis         histopathology   Paigen1 fatty streak aortic lesion size   [µm2] ...... 0   0 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 fatty streak aortic lesion size   [µm2] ...... 0   128     high-fat diet   17wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — insulin         ELISA   Naggert1 insulin   [ng/mL] ...... 0.731   0.994     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — leptin         ELISA   Naggert1 leptin   [pg/mL] ...... 9614   3443     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   gallbladder — pathology and gallstones         gravimetry   Paigen1 gallbladder volume   [µL] ...... 17.0   23.6 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred   gallbladder — pathology and gallstones         histopathology   Paigen1 gallbladder pathology (0-4) [score]   high-fat diet   8wks       • mucin  ...... 0.889 ▼   1.33 (N=3)         • arc  ...... 0   0 (N=3)         • filaments  ...... 0   0 (N=3)               liquid crystals:             • small  ...... 1.11   0.500 (N=3) ▼               • aggregated  ...... 1.17   0.500 (N=3)               • fused  ...... 0.778   0.500 (N=3)                 • comparison         cholesterol monohydrate crystals:             • all  ...... 0   2.67 (N=3) ▲               43 strains   age 15-19wks   Inbred Paigen1 gallstones [n]   high-fat diet   8wks       • sandy  ...... 0   7.00 (N=3) ▲         • true  ...... 0   0 (N=3)         • all  ...... 0   7.00 (N=3)         • presence score [0/1]  ...... 0   1.00 (N=3) ▲▲           • comparison   43 strains   age 15-19wks   Inbred   kidney — function — urinalysis         spectrophotometry   Rusyn5 urine alcohol concentration (UAC), daily average [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 15.5 (N=3)         • high-fat and ethanol  ...... 97.4 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 urine alcohol concentration (UAC), daily peak [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 46.1 (N=3)         • high-fat and ethanol  ...... 419 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — gene expression         RT-PCR   Rusyn5 Acox1 [%]   high-fat diet, ethanol   gene: Acox1         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Adipor2 [%]   high-fat diet, ethanol   gene: Adipor2         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Ahcy [%]   high-fat diet, ethanol   gene: Ahcy         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 67.6 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cbs [%]   high-fat diet, ethanol   gene: Cbs         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 27.0 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cebpa [%]   high-fat diet, ethanol   gene: Cebpa         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 73.9 (N=2)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cth [%]   high-fat diet, ethanol   gene: Cth         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 60.3 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Ddit3 (Chop) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 171 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Gnmt [%]   high-fat diet, ethanol   gene: Gnmt         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 48.0 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mat1a [%]   high-fat diet, ethanol   gene: Mat1a         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 97.7 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mthfr [%]   high-fat diet, ethanol   gene: Mthfr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 33.4 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mtr [%]   high-fat diet, ethanol   gene: Mtr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 47.3 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mttp [%]   high-fat diet, ethanol   gene: Mttp         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Srebf1 [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 46.1 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — lipid homeostasis         lipid profile   Paigen1 hepatic cholesterol concentration [mg]   high-fat diet   8wks       • free  ...... 3.09 ▼   5.74 (N=3)         • esterified  ...... 32.6   36.6 (N=3)         • total  ...... 35.1   42.4 (N=3)         • free to esterified ratio  ...... 0.102   0.183 (N=3)         43 strains   age 15-19wks   Inbred Rusyn5 hepatic triglycerides (TG) [mg/g]   high-fat diet, ethanol       • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred   liver — pathology         histopathology   Paigen1 liver pathology (0-4) [score]   high-fat diet   8wks       • images  ......       • microvacuoles  ...... 4.00 (N=2) ▲   2.00 (N=2)         • macrovacuoles  ...... 0 (N=2)   0 (N=2)         • inflammation  ...... 1.50 (N=2) ▲   0 (N=2)         43 strains   age 15-19wks   Inbred Rusyn5 liver steatosis (0-4) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 1.33 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver inflammation (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 1.00 (N=3)         • high-fat and ethanol  ...... 2.00 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver necrosis (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 1.00 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 total liver histopathology (0-8) [score]   high-fat diet, ethanol       • high-fat only  ...... 1.00 (N=3)         • high-fat and ethanol  ...... 4.33 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         HPLC   Rusyn5 liver homocysteine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.217 (N=3)         • high-fat and ethanol  ...... 0.257 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylhomocysteine (SAH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.223 (N=3)         • high-fat and ethanol  ...... 0.367 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver methionine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.760 (N=3)         • high-fat and ethanol  ...... 0.950 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylmethionine (SAM) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.533 (N=3) ▼         • high-fat and ethanol  ...... 0.593 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of liver SAM to SAH (SAM/SAH) [ratio]   high-fat diet, ethanol       • high-fat only  ...... 2.42 (N=3)         • high-fat and ethanol  ...... 1.60 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         Western blot   Rusyn5 BHMT [%]   high-fat diet, ethanol   gene: Bhmt         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 166 (N=2) ▲▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 DDIT3 (CHOP) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 526 (N=2) ▲▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 HSPA5 (GRP78) [%]   high-fat diet, ethanol   gene: Hspa5         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 101 (N=2)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 SREBF1 (NSREBP1) [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 70.5 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis — glutathione         HPLC   Rusyn5 reduced liver glutathione (GSH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 43.6 (N=3)         • high-fat and ethanol  ...... 23.0 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 oxidized liver glutathione (GSSG) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 1.50 (N=3)         • high-fat and ethanol  ...... 1.77 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of reduced to oxidized liver glutathione [ratio]   high-fat diet, ethanol       • high-fat only  ...... 30.9 (N=3)         • high-fat and ethanol  ...... 12.7 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — weight         scales   Paigen1 liver weight at sacrifice   [g] ...... 2.20   1.96 (N=3)     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Rusyn5 liver weight as a percent of body weight [%]   high-fat diet, ethanol       • high-fat only  ...... 6.72 (N=3)         • high-fat and ethanol  ...... 8.19 (N=3)           • comparison   14 strains   age 8-14wks   Inbred

128 measurements listed.