blood — clinical chemistry — electrolytes — calcium         metabolic panel   Shockley1 calcium (Ca) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 2.32 ▼   2.58 ▼         • high-fat diet  ...... 2.40 (N=3) ▼   2.85 (N=4)         • difference  ...... 1.03   1.10           • comparison   10 strains   age 10-13wks   Inbred   blood — clinical chemistry — enzymes — ALT         metabolic panel   Paigen1 alanine aminotransferase (plasma ALT)   [IU/L] ...... 56.5   127     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen3 alanine aminotransferase (plasma ALT)   [IU/L] ...... 56.5   127     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants   blood — clinical chemistry — enzymes — misc         metabolic panel   Shockley1 glutamate dehydrogenase (GLDH) [IU/L]     control and  high-fat diet 4wks       • control diet  ...... 9.68   9.20 ▼         • high-fat diet  ...... 102   76.9         • difference  ...... 10.5 ▲   8.36           • comparison   10 strains   age 10-13wks   Inbred   blood — clinical chemistry — glucose         metabolic panel   Naggert1 glucose (plasma GLU, 4h fast) [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 224 ▲   218         • after 17wks on diet  ...... 190 ▲   199         • Δchange  ...... 0.857   0.861           • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 glucose (GLU) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 130   160 ▲         • high-fat diet  ...... 140 (N=4) ▲   140         • difference  ...... 1.08   0.875           • comparison   10 strains   age 10-13wks   Inbred   blood — clinical chemistry — miscellaneous         metabolic panel   Paigen1 bile salts (plasma)   [µmol/L] ...... 51.9   68.4     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen3 bile salts (plasma)   [µmol/L] ...... 51.9   68.4     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants   blood — clinical chemistry — urea nitrogen         metabolic panel   Shockley1 blood urea nitrogen (BUN) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 22.8 ▲   24.6 ▲         • high-fat diet  ...... 26.0 (N=1) ▲         • difference  ...... 1.14           • comparison   10 strains   age 10-13wks   Inbred   blood — lipids — cholesterol — HDL         lipid profile   Albers1 HDL cholesterol [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.12   1.60         • after 6wks on diet  ...... 0.860   1.46         • Δchange  ...... 0.770 ▼   0.910           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 percentage of large HDL particles (10-13 nm) [%]     baseline and  high-fat diet 6wks       • baseline  ...... 44.6   44.6         • after 6wks on diet  ...... 34.3   35.9         • Δchange  ...... 0.770   0.800           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 HDL peak size [nm]     baseline and  high-fat diet 6wks       • baseline  ...... 9.67   9.56         • after 6wks on diet  ...... 9.21   9.30         • Δchange  ...... 0.952 ▼   0.973 ▼           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen1 HDL cholesterol (plasma)   high-fat diet   8wks       • amount  ...... 53.9   61.3         • % of total plasma cholesterol  ...... 28.7   23.8 ▼         43 strains   age 15-19wks   Inbred Paigen2 HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 49.7   72.3         • after 17wks on diet  ...... 47.1 ▼   55.6 ▼         • Δchange  ...... 0.952   0.769 ▼           • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 HDL cholesterol (HDL) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 70.6   87.2         • high-fat diet  ...... 64.4   94.5         • difference  ...... 0.912 ▼   1.08           • comparison   10 strains   age 10-13wks   Inbred Paigen3 HDL cholesterol (plasma)   high-fat diet   8wks       • amount  ...... 53.9   61.3         • % of total plasma cholesterol  ...... 28.7   23.8         8 strains   age 15-19wks   obesity mutants   blood — lipids — cholesterol — LDL or non-HDL         lipid profile   Albers1 non-HDL cholesterol [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 0.340   0.260 ▼         • after 6wks on diet  ...... 6.45   7.51 ▲         • Δchange  ...... 19.0 ▲   28.9 ▲▲           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen1 plasma cholesterol not in the HDL fraction   [mg/dL] ...... 124   205     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 non-HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 33.4   44.7 ▲         • after 17wks on diet  ...... 152   216         • Δchange  ...... 5.73   5.89           • comparison   43 strains   age 7-9wks                 at baseline   Inbred Paigen3 plasma cholesterol not in the HDL fraction   [mg/dL] ...... 124   205     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants   blood — lipids — cholesterol — total         lipid profile   Albers1 total cholesterol [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.46   1.85         • after 6wks on diet  ...... 7.31   8.97         • Δchange  ...... 5.01   4.85 ▲           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen1 total cholesterol (plasma)   [mg/dL] ...... 173   267     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 total cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 83.1   117         • after 17wks on diet  ...... 200   272         • Δchange  ...... 2.54   2.43           • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 total cholesterol (CHOL) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 74.4   89.6         • high-fat diet  ...... 157   191         • difference  ...... 2.11 ▼   2.13           • comparison   10 strains   age 10-13wks   Inbred Paigen3 total cholesterol (plasma)   [mg/dL] ...... 173   267     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants   blood — lipids — fatty acids         lipid profile   Shockley1 nonesterified fatty acid (NEFA) [mEq/L]     control and  high-fat diet 4wks       • control diet  ...... 2.06   2.10         • high-fat diet  ...... 1.62 ▼   1.56 ▼         • difference  ...... 0.786   0.743           • comparison   10 strains   age 10-13wks   Inbred   blood — lipids — phospholipid         lipid profile   Albers1 total phospholipid [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.82   2.38         • after 6wks on diet  ...... 1.94   2.98         • Δchange  ...... 1.07   1.25           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 HDL phospholipid [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.56   2.11         • after 6wks on diet  ...... 0.820 ▼   1.40         • Δchange  ...... 0.530 ▼   0.660           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 non-HDL phospholipid [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 0.260   0.270         • after 6wks on diet  ...... 1.12   1.58         • Δchange  ...... 4.31   5.85 ▲           • comparison   15 strains   age 6-8wks                 at baseline   Inbred   blood — lipids — phospholipid — transfer activity         radiolabeling   Albers1 phospholipid transfer protein activity [µmol/mL/h]     baseline and  high-fat diet 6wks       • baseline  ...... 29.4 ▲   31.8 ▲         • after 6wks on diet  ...... 39.0   45.7         • Δchange  ...... 1.33 ▼   1.44           • comparison   15 strains   age 6-8wks                 at baseline   Inbred   blood — lipids — triglycerides         lipid profile   Albers1 triglycerides [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 0.880 ▼   0.940 ▼         • after 6wks on diet  ...... 0.840   0.760 ▼         • Δchange  ...... 0.950   0.810 ▲           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen2 triglycerides [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 72.8   100         • after 17wks on diet  ...... 43.2   61.2         • Δchange  ...... 0.599   0.637           • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 triglycerides (TG) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 66.4   65.6         • high-fat diet  ...... 51.0   45.2         • difference  ...... 0.768   0.689           • comparison   10 strains   age 10-13wks   Inbred   body composition — fat — percent         DXA   Naggert1 percent of tissue mass that is fat, head excluded   [%] ...... 17.2 ▼   13.3 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — fat — weight         DXA   Naggert1 weight of fat portion of tissue mass, head excluded   [g] ...... 3.42   3.17 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — lean         DXA   Naggert1 weight of lean portion of tissue mass, head excluded   [g] ...... 16.4   20.7     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — total         DXA   Naggert1 total tissue mass, head excluded   [g] ...... 19.9   23.9     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body weight         scales   Naggert1 body weight   [g] ...... 20.7   25.0     high-fat diet   8wks 43 strains   age 15-17wks   Inbred Paigen1 body weight [g]     baseline and  high-fat diet 8wks       • start  ...... 19.3   25.9         • after 8wks on diet  ...... 21.5   25.2         • Δchange  ...... 1.12   0.976 ▼           • comparison   43 strains   age 7-11wks                 at baseline   Inbred Shockley1 body weight [g]     control and  high-fat diet 4wks       • control diet  ...... 18.0   25.3         • high-fat diet  ...... 18.7   25.1         • difference  ...... 1.04   0.992           • comparison   10 strains   age 10-13wks   Inbred Nadeau1 body weight [g]     baseline and  high-fat diet 12wks       • baseline  ...... 24.6         • after 12wks on diet  ...... 42.7 ▲         • Δchange  ...... 1.73 ▲           • comparison   24 strains   age 5wks                 at baseline   B6.A consomic Paigen3 body weight [g]     baseline and  high-fat diet 8wks       • start  ...... 19.3   25.9         • after 8wks on diet  ...... 21.5   25.2         • Δchange  ...... 1.12   0.976           • comparison   8 strains   age 7-11wks                 at baseline   obesity mutants   bone — mineral density and content — whole body without head         DXA   Naggert1 bone mineral density and content   high-fat diet   8wks       • BMD  ...... 0.0451   0.0468         • BMC  ...... 0.407   0.463         43 strains   age 15-17wks   Inbred   cardiovascular — atherosclerosis         histopathology   Paigen1 fatty streak aortic lesion size   [µm2] ...... 1505 ▲▲   3428 ▲▲     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 fatty streak aortic lesion size   [µm2] ...... 41.2   97.0     high-fat diet   17wks 43 strains   age 25-27wks   Inbred Paigen3 fatty streak aortic lesion size   [µm2] ...... 1505   3428     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants   endocrine — hormones — insulin         ELISA   Naggert1 insulin   [ng/mL] ...... 0.837   0.639 ▼     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — leptin         ELISA   Naggert1 leptin   [pg/mL] ...... 5054   3600     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — thyroxine         metabolic panel   Shockley1 thyroxine (T4) [µg/dL]     control and  high-fat diet 4wks       • control diet  ...... 5.06   5.24         • high-fat diet  ...... 7.23 (N=3)   4.70 (N=2)         • difference  ...... 1.43   0.897           • comparison   10 strains   age 10-13wks   Inbred   gallbladder — pathology and gallstones         gravimetry   Paigen1 gallbladder volume   [µL] ...... 23.6   28.7     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen3 gallbladder volume   [µL] ...... 23.6   28.7     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants   gallbladder — pathology and gallstones         histopathology   Paigen1 gallbladder pathology (0-4) [score]   high-fat diet   8wks       • mucin  ...... 1.90   1.58         • arc  ...... 0   0         • filaments  ...... 0   0               liquid crystals:             • small  ...... 2.50 ▲   1.17               • aggregated  ...... 1.30   1.00               • fused  ...... 0.900   0.333                 • comparison         cholesterol monohydrate crystals:             • all  ...... 0.150   2.50 ▲               43 strains   age 15-19wks   Inbred Paigen1 gallstones [n]   high-fat diet   8wks       • sandy  ...... 0.200   2.17         • true  ...... 0   2.50         • all  ...... 0.200   4.67         • presence score [0/1]  ...... 0.100   0.688 ▲           • comparison   43 strains   age 15-19wks   Inbred Paigen3 gallbladder pathology (0-4) [score]   high-fat diet   8wks       • mucin  ...... 1.90   1.58         • arc  ...... 0   0         • filaments  ...... 0   0               liquid crystals:             • small  ...... 2.50 ▲   1.17               • aggregated  ...... 1.30 ▲   1.00 ▲               • fused  ...... 0.900 ▲   0.333                 • comparison         cholesterol monohydrate crystals:             • all  ...... 0.150   2.50 ▲               8 strains   age 15-19wks   obesity mutants Paigen3 gallstones [n]   high-fat diet   8wks       • sandy  ...... 0.200   2.17         • true  ...... 0   2.50         • all  ...... 0.200   4.67         • presence score [0/1]  ...... 0.100   0.688 ▲           • comparison   8 strains   age 15-19wks   obesity mutants   liver — lipid homeostasis         lipid profile   Paigen1 hepatic cholesterol concentration [mg]   high-fat diet   8wks       • free  ...... 4.80   5.99         • esterified  ...... 37.2   39.5         • total  ...... 42.3   41.3         • free to esterified ratio  ...... 0.136   0.153         43 strains   age 15-19wks   Inbred Paigen3 hepatic cholesterol concentration [mg]   high-fat diet   8wks       • free  ...... 4.80   5.99         • esterified  ...... 37.2   39.5         • total  ...... 42.3   41.3         • free to esterified ratio  ...... 0.136   0.153 ▼         8 strains   age 15-19wks   obesity mutants   liver — pathology         histopathology   Paigen1 liver pathology (0-4) [score]   high-fat diet   8wks       • images  ......       • microvacuoles  ...... 3.00 (N=3)   2.67 (N=3)         • macrovacuoles  ...... 0 (N=3)   0 (N=3)         • inflammation  ...... 1.00 (N=3)   1.33 (N=3)         43 strains   age 15-19wks   Inbred Paigen3 liver pathology (0-4) [score]   high-fat diet   8wks       • images  ......       • microvacuoles  ...... 3.00 (N=3)   2.67 (N=3)         • macrovacuoles  ...... 0 (N=3)   0 (N=3)         • inflammation  ...... 1.00 (N=3)   1.33 (N=3)         8 strains   age 15-19wks   obesity mutants   liver — weight         scales   Paigen1 liver weight at sacrifice   [g] ...... 1.54   1.76     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen3 liver weight at sacrifice   [g] ...... 1.54   1.76     high-fat diet   8wks 8 strains   age 15-19wks   obesity mutants

150 measurements listed.