blood — clinical chemistry — electrolytes — calcium         metabolic panel   Shockley1 calcium (Ca) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 2.60   3.46         • high-fat diet  ...... 3.98 ▲   4.16 ▲         • difference  ...... 1.53 ▲   1.20           • comparison   10 strains   age 10-13wks   Inbred   blood — clinical chemistry — enzymes — ALT         metabolic panel   Paigen1 alanine aminotransferase (plasma ALT)   [IU/L] ...... 106   280 ▲     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Rusyn5 serum alanine aminotransferase (ALT) [IU/L]   high-fat diet, ethanol       • high-fat only  ...... 21.4 (N=3)         • high-fat and ethanol  ...... 43.7 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   blood — clinical chemistry — enzymes — misc         metabolic panel   Shockley1 glutamate dehydrogenase (GLDH) [IU/L]     control and  high-fat diet 4wks       • control diet  ...... 14.5   14.0         • high-fat diet  ...... 87.5   354         • difference  ...... 6.03   25.3           • comparison   10 strains   age 10-13wks   Inbred   blood — clinical chemistry — glucose         metabolic panel   Naggert1 glucose (plasma GLU, 4h fast) [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 146   158         • after 17wks on diet  ...... 106 ▼   124         • Δchange  ...... 0.725   0.795           • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 glucose (GLU) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 105   98.6 ▼         • high-fat diet  ...... 93.4   76.0 ▼         • difference  ...... 0.890   0.771 ▼           • comparison   10 strains   age 10-13wks   Inbred   blood — clinical chemistry — miscellaneous         metabolic panel   Paigen1 bile salts (plasma)   [µmol/L] ...... 54.9   53.2     high-fat diet   8wks 43 strains   age 15-19wks   Inbred   blood — clinical chemistry — proteins — misc         HPLC   Rusyn5 homocysteine concentration (plasma) [nmol/mL]   high-fat diet, ethanol       • high-fat only  ...... 5.98 (N=3)         • high-fat and ethanol  ...... 19.9 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   blood — clinical chemistry — urea nitrogen         metabolic panel   Shockley1 blood urea nitrogen (BUN) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 14.6   17.0 ▼         • high-fat diet  ...... 18.6   20.0         • difference  ...... 1.27   1.18           • comparison   10 strains   age 10-13wks   Inbred   blood — lipids — cholesterol — HDL         lipid profile   Albers1 HDL cholesterol [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.39   1.72         • after 6wks on diet  ...... 1.55   2.07         • Δchange  ...... 1.12   1.20           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 percentage of large HDL particles (10-13 nm) [%]     baseline and  high-fat diet 6wks       • baseline  ...... 62.8 ▲   41.5         • after 6wks on diet  ...... 51.9   44.3         • Δchange  ...... 0.830   1.07           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 HDL peak size [nm]     baseline and  high-fat diet 6wks       • baseline  ...... 9.93 ▲   9.70         • after 6wks on diet  ...... 9.89 ▲   9.52         • Δchange  ...... 0.996   0.981           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen1 HDL cholesterol (plasma)   high-fat diet   8wks       • amount  ...... 112   68.0         • % of total plasma cholesterol  ...... 62.1 ▲   55.8         43 strains   age 15-19wks   Inbred Paigen2 HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ......       • after 17wks on diet  ......       • Δchange  ......         • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 HDL cholesterol (HDL) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 77.0   92.9         • high-fat diet  ...... 94.1   95.8         • difference  ...... 1.22   1.03 ▼           • comparison   10 strains   age 10-13wks   Inbred   blood — lipids — cholesterol — LDL or non-HDL         lipid profile   Albers1 non-HDL cholesterol [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 0.380   0.230 ▼         • after 6wks on diet  ...... 3.57   2.07 ▼         • Δchange  ...... 9.39   9.00           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen1 plasma cholesterol not in the HDL fraction   [mg/dL] ...... 69.7   55.9     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 non-HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ......       • after 17wks on diet  ......       • Δchange  ......         • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — cholesterol — total         lipid profile   Albers1 total cholesterol [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.77   1.95         • after 6wks on diet  ...... 5.12   4.14 ▼         • Δchange  ...... 2.89   2.12 ▼           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen1 total cholesterol (plasma)   [mg/dL] ...... 182   124 ▼     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 total cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ......       • after 17wks on diet  ......       • Δchange  ......         • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 total cholesterol (CHOL) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 73.4   68.4 ▼         • high-fat diet  ...... 175   132 ▼         • difference  ...... 2.38   1.93           • comparison   10 strains   age 10-13wks   Inbred   blood — lipids — fatty acids         lipid profile   Shockley1 nonesterified fatty acid (NEFA) [mEq/L]     control and  high-fat diet 4wks       • control diet  ...... 2.15   2.03         • high-fat diet  ...... 2.76   2.57         • difference  ...... 1.28 ▲   1.27 ▲           • comparison   10 strains   age 10-13wks   Inbred   blood — lipids — phospholipid         lipid profile   Albers1 total phospholipid [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.94   2.34         • after 6wks on diet  ...... 2.24   2.49 ▼         • Δchange  ...... 1.15   1.06           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 HDL phospholipid [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.82   2.18         • after 6wks on diet  ...... 1.58   1.86         • Δchange  ...... 0.870   0.850           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Albers1 non-HDL phospholipid [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 0.110 ▼   0.160 ▼         • after 6wks on diet  ...... 0.660   0.630 ▼         • Δchange  ...... 6.00   3.94           • comparison   15 strains   age 6-8wks                 at baseline   Inbred   blood — lipids — phospholipid — transfer activity         radiolabeling   Albers1 phospholipid transfer protein activity [µmol/mL/h]     baseline and  high-fat diet 6wks       • baseline  ...... 19.2   23.6         • after 6wks on diet  ...... 34.1   39.4         • Δchange  ...... 1.78   1.67           • comparison   15 strains   age 6-8wks                 at baseline   Inbred   blood — lipids — triglycerides         lipid profile   Albers1 triglycerides [mmol/L]     baseline and  high-fat diet 6wks       • baseline  ...... 1.39   1.48         • after 6wks on diet  ...... 0.820   0.790 ▼         • Δchange  ...... 0.590   0.530           • comparison   15 strains   age 6-8wks                 at baseline   Inbred Paigen2 triglycerides [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ......       • after 17wks on diet  ......       • Δchange  ......         • comparison   43 strains   age 7-9wks                 at baseline   Inbred Shockley1 triglycerides (TG) [mg/dL]     control and  high-fat diet 4wks       • control diet  ...... 62.6   51.6 ▼         • high-fat diet  ...... 55.8   49.6         • difference  ...... 0.891   0.961 ▲           • comparison   10 strains   age 10-13wks   Inbred   blood — xenobiotics — alcohol concentration         spectrophotometry   Rusyn5 blood alcohol concentration (BAC) [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 31.8 (N=3)         • high-fat and ethanol  ...... 52.8 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred   body composition — fat — percent         DXA   Naggert1 percent of tissue mass that is fat, head excluded   [%] ...... 25.4   18.9     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — fat — weight         DXA   Naggert1 weight of fat portion of tissue mass, head excluded   [g] ...... 6.02   5.35     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — lean         DXA   Naggert1 weight of lean portion of tissue mass, head excluded   [g] ...... 17.6   22.9     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — total         DXA   Naggert1 total tissue mass, head excluded   [g] ...... 23.6   28.3     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body weight         scales   Naggert1 body weight   [g] ...... 24.7   29.2     high-fat diet   8wks 43 strains   age 15-17wks   Inbred Paigen1 body weight [g]     baseline and  high-fat diet 8wks       • start  ...... 20.8   25.7         • after 8wks on diet  ...... 26.2   32.0         • Δchange  ...... 1.26   1.25           • comparison   43 strains   age 7-11wks                 at baseline   Inbred Shockley1 body weight [g]     control and  high-fat diet 4wks       • control diet  ...... 19.5   24.0         • high-fat diet  ...... 22.4   27.8         • difference  ...... 1.15   1.16           • comparison   10 strains   age 10-13wks   Inbred   bone — mineral density and content — whole body without head         DXA   Naggert1 bone mineral density and content   high-fat diet   8wks       • BMD  ...... 0.0525   0.0513         • BMC  ...... 0.507   0.533         43 strains   age 15-17wks   Inbred   cardiovascular — atherosclerosis         histopathology   Paigen1 fatty streak aortic lesion size   [µm2] ...... 0   0     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 fatty streak aortic lesion size   [µm2] ...... 982   105     high-fat diet   17wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — insulin         ELISA   Naggert1 insulin   [ng/mL] ...... 1.19   0.938     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — leptin         ELISA   Naggert1 leptin   [pg/mL] ...... 13548   2966     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — thyroxine         metabolic panel   Shockley1 thyroxine (T4) [µg/dL]     control and  high-fat diet 4wks       • control diet  ...... 6.76   5.58         • high-fat diet  ...... 12.3 ▲   6.62         • difference  ...... 1.82 ▲   1.19           • comparison   10 strains   age 10-13wks   Inbred   gallbladder — pathology and gallstones         gravimetry   Paigen1 gallbladder volume   [µL] ...... 7.22   7.91     high-fat diet   8wks 43 strains   age 15-19wks   Inbred   gallbladder — pathology and gallstones         histopathology   Paigen1 gallbladder pathology (0-4) [score]   high-fat diet   8wks       • mucin  ...... 0.800 ▼   1.75         • arc  ...... 0   0         • filaments  ...... 0.200 ▲▲   0               liquid crystals:             • small  ...... 0.750   1.00               • aggregated  ...... 0.350 ▼   0.350               • fused  ...... 0.200 ▼   0.0500 ▼                 • comparison         cholesterol monohydrate crystals:             • all  ...... 0.100   1.70               43 strains   age 15-19wks   Inbred Paigen1 gallstones [n]   high-fat diet   8wks       • sandy  ...... 0   1.00         • true  ...... 0.100   0.100         • all  ...... 0.100   1.10         • presence score [0/1]  ...... 0.100   0.400           • comparison   43 strains   age 15-19wks   Inbred   kidney — function — urinalysis         spectrophotometry   Rusyn5 urine alcohol concentration (UAC), daily average [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 28.7 (N=3) ▲         • high-fat and ethanol  ...... 101 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 urine alcohol concentration (UAC), daily peak [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 65.7 (N=3)         • high-fat and ethanol  ...... 337 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — gene expression         RT-PCR   Rusyn5 Acox1 [%]   high-fat diet, ethanol   gene: Acox1         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Adipor2 [%]   high-fat diet, ethanol   gene: Adipor2         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Ahcy [%]   high-fat diet, ethanol   gene: Ahcy         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 185 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cbs [%]   high-fat diet, ethanol   gene: Cbs         • high-fat only  ...... 100.0 (N=3)         • high-fat and ethanol  ...... 101 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cebpa [%]   high-fat diet, ethanol   gene: Cebpa         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 78.8 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cth [%]   high-fat diet, ethanol   gene: Cth         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 124 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Ddit3 (Chop) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 152 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Gnmt [%]   high-fat diet, ethanol   gene: Gnmt         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 136 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mat1a [%]   high-fat diet, ethanol   gene: Mat1a         • high-fat only  ...... 100.0 (N=3)         • high-fat and ethanol  ...... 197 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mthfr [%]   high-fat diet, ethanol   gene: Mthfr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 47.3 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mtr [%]   high-fat diet, ethanol   gene: Mtr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 72.6 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mttp [%]   high-fat diet, ethanol   gene: Mttp         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Srebf1 [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 120 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — lipid homeostasis         lipid profile   Paigen1 hepatic cholesterol concentration [mg]   high-fat diet   8wks       • free  ...... 2.72 ▼   6.00         • esterified  ...... 28.1   25.7         • total  ...... 33.7   35.4         • free to esterified ratio  ...... 0.102   0.277         43 strains   age 15-19wks   Inbred Rusyn5 hepatic triglycerides (TG) [mg/g]   high-fat diet, ethanol       • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred   liver — pathology         histopathology   Paigen1 liver pathology (0-4) [score]   high-fat diet   8wks       • images  ......       • microvacuoles  ...... 3.00 (N=3)   3.33 (N=3)         • macrovacuoles  ...... 0 (N=3)   0 (N=3)         • inflammation  ...... 0.667 (N=3)   0 (N=3)         43 strains   age 15-19wks   Inbred Rusyn5 liver steatosis (0-4) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 1.50 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver inflammation (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 1.17 (N=3)         • high-fat and ethanol  ...... 1.00 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver necrosis (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 1.00 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 total liver histopathology (0-8) [score]   high-fat diet, ethanol       • high-fat only  ...... 1.17 (N=3)         • high-fat and ethanol  ...... 3.50 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         HPLC   Rusyn5 liver homocysteine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.180 (N=3) ▼         • high-fat and ethanol  ...... 0.237 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylhomocysteine (SAH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.190 (N=3) ▼         • high-fat and ethanol  ...... 0.310 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver methionine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.790 (N=3)         • high-fat and ethanol  ...... 0.713 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylmethionine (SAM) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.577 (N=3)         • high-fat and ethanol  ...... 0.483 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of liver SAM to SAH (SAM/SAH) [ratio]   high-fat diet, ethanol       • high-fat only  ...... 3.08 (N=3)         • high-fat and ethanol  ...... 1.57 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         Western blot   Rusyn5 BHMT [%]   high-fat diet, ethanol   gene: Bhmt         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 89.0 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 DDIT3 (CHOP) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 146 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 HSPA5 (GRP78) [%]   high-fat diet, ethanol   gene: Hspa5         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 93.9 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 SREBF1 (NSREBP1) [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 61.8 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis — glutathione         HPLC   Rusyn5 reduced liver glutathione (GSH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 52.6 (N=3)         • high-fat and ethanol  ...... 35.4 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 oxidized liver glutathione (GSSG) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 1.82 (N=3)         • high-fat and ethanol  ...... 2.38 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of reduced to oxidized liver glutathione [ratio]   high-fat diet, ethanol       • high-fat only  ...... 30.2 (N=3)         • high-fat and ethanol  ...... 15.7 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — weight         scales   Paigen1 liver weight at sacrifice   [g] ...... 1.95   2.23     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Rusyn5 liver weight as a percent of body weight [%]   high-fat diet, ethanol       • high-fat only  ...... 6.53 (N=3)         • high-fat and ethanol  ...... 8.23 (N=3)           • comparison   14 strains   age 8-14wks   Inbred

188 measurements listed.