blood — clinical chemistry — enzymes — ALT         metabolic panel   Paigen1 alanine aminotransferase (plasma ALT)   [IU/L] ...... 162   520 ▲▲     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Rusyn5 serum alanine aminotransferase (ALT) [IU/L]   high-fat diet, ethanol       • high-fat only  ...... 43.9 (N=3) ▲         • high-fat and ethanol  ...... 46.2 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   blood — clinical chemistry — glucose         metabolic panel   Naggert1 glucose (plasma GLU, 4h fast) [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 108 ▼▼   144 ▼         • after 17wks on diet  ...... 127   105 ▼         • Δchange  ...... 1.68 ▲▲   0.677 ▼           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — clinical chemistry — miscellaneous         metabolic panel   Paigen1 bile salts (plasma)   [µmol/L] ...... 57.0   72.3     high-fat diet   8wks 43 strains   age 15-19wks   Inbred   blood — clinical chemistry — proteins — misc         HPLC   Rusyn5 homocysteine concentration (plasma) [nmol/mL]   high-fat diet, ethanol       • high-fat only  ...... 2.89 (N=2) ▼         • high-fat and ethanol  ...... 8.29 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   blood — lipids — cholesterol — HDL         lipid profile   Paigen1 HDL cholesterol (plasma)   high-fat diet   8wks       • amount  ...... 48.2   88.0         • % of total plasma cholesterol  ...... 22.3 ▼   37.2         43 strains   age 15-19wks   Inbred Paigen2 HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 66.5   92.3         • after 17wks on diet  ...... 92.8   96.0         • Δchange  ...... 1.42   1.02           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — cholesterol — LDL or non-HDL         lipid profile   Paigen1 plasma cholesterol not in the HDL fraction   [mg/dL] ...... 185   149     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 non-HDL cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 8.62 ▼   10.6 ▼         • after 17wks on diet  ...... 190   148         • Δchange  ...... 50.2 ▲▲   28.6 ▲           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — cholesterol — total         lipid profile   Paigen1 total cholesterol (plasma)   [mg/dL] ...... 235   237     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 total cholesterol [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 68.2   102         • after 17wks on diet  ...... 282   244         • Δchange  ...... 7.44 ▲   2.34           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — lipids — triglycerides         lipid profile   Paigen2 triglycerides [mg/dL]     baseline and  high-fat diet 17wks       • baseline  ...... 79.9   93.9         • after 17wks on diet  ...... 82.6   62.1         • Δchange  ...... 1.08   0.818           • comparison   43 strains   age 7-9wks                 at baseline   Inbred   blood — xenobiotics — alcohol concentration         spectrophotometry   Rusyn5 blood alcohol concentration (BAC) [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 52.5 (N=3) ▲         • high-fat and ethanol  ...... 92.8 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred   body composition — fat — percent         DXA   Naggert1 percent of tissue mass that is fat, head excluded   [%] ...... 13.7 ▼   10.8 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — fat — weight         DXA   Naggert1 weight of fat portion of tissue mass, head excluded   [g] ...... 1.71 ▼   1.58 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — lean         DXA   Naggert1 weight of lean portion of tissue mass, head excluded   [g] ...... 10.8 ▼   13.0 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body composition — total         DXA   Naggert1 total tissue mass, head excluded   [g] ...... 12.5 ▼   14.6 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred   body weight         scales   Naggert1 body weight   [g] ...... 13.3 ▼   15.3 ▼     high-fat diet   8wks 43 strains   age 15-17wks   Inbred Paigen1 body weight [g]     baseline and  high-fat diet 8wks       • start  ......       • after 8wks on diet  ...... 11.7 ▼▼   14.3 ▼▼         • Δchange  ......         • comparison   43 strains   age 7-11wks                 at baseline   Inbred   bone — mineral density and content — whole body without head         DXA   Naggert1 bone mineral density and content   high-fat diet   8wks       • BMD  ...... 0.0399 ▼   0.0411 ▼         • BMC  ...... 0.300 ▼   0.341 ▼         43 strains   age 15-17wks   Inbred   cardiovascular — atherosclerosis         histopathology   Paigen1 fatty streak aortic lesion size   [µm2] ...... 0   0     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Paigen2 fatty streak aortic lesion size   [µm2] ...... 47.2   0     high-fat diet   17wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — insulin         ELISA   Naggert1 insulin   [ng/mL] ...... 0.790   0.804     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   endocrine — hormones — leptin         ELISA   Naggert1 leptin   [pg/mL] ...... 3581 ▼   2404 ▼     high-fat diet   18wks 43 strains   age 25-27wks   Inbred   gallbladder — pathology and gallstones         gravimetry   Paigen1 gallbladder volume   [µL] ...... 2.31 ▼   1.58 ▼     high-fat diet   8wks 43 strains   age 15-19wks   Inbred   gallbladder — pathology and gallstones         histopathology   Paigen1 gallbladder pathology (0-4) [score]   high-fat diet   8wks       • mucin  ...... 2.25 ▲   2.60 ▲         • arc  ...... 0   0         • filaments  ...... 0   0               liquid crystals:             • small  ...... 1.19   1.17               • aggregated  ...... 0.625   0.833               • fused  ...... 0.500   0.667                 • comparison         cholesterol monohydrate crystals:             • all  ...... 0.688   0.917               43 strains   age 15-19wks   Inbred Paigen1 gallstones [n]   high-fat diet   8wks       • sandy  ...... 0.500   0.167         • true  ...... 0   0         • all  ...... 0.500   0.167         • presence score [0/1]  ...... 0.250   0.167           • comparison   43 strains   age 15-19wks   Inbred   kidney — function — urinalysis         spectrophotometry   Rusyn5 urine alcohol concentration (UAC), daily average [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 31.3 (N=3) ▲         • high-fat and ethanol  ...... 146 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 urine alcohol concentration (UAC), daily peak [mg/dL]   high-fat diet, ethanol       • high-fat only  ...... 95.1 (N=3)         • high-fat and ethanol  ...... 395 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — gene expression         RT-PCR   Rusyn5 Acox1 [%]   high-fat diet, ethanol   gene: Acox1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 96.4 (N=3)           • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Adipor2 [%]   high-fat diet, ethanol   gene: Adipor2         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 155 (N=3) ▲           • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Ahcy [%]   high-fat diet, ethanol   gene: Ahcy         • high-fat only  ...... 100.0 (N=3)         • high-fat and ethanol  ...... 187 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cbs [%]   high-fat diet, ethanol   gene: Cbs         • high-fat only  ...... 100.0 (N=3)         • high-fat and ethanol  ...... 113 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cebpa [%]   high-fat diet, ethanol   gene: Cebpa         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 411 (N=3) ▲▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cth [%]   high-fat diet, ethanol   gene: Cth         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 211 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Ddit3 (Chop) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 120 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Gnmt [%]   high-fat diet, ethanol   gene: Gnmt         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 205 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mat1a [%]   high-fat diet, ethanol   gene: Mat1a         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 197 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mthfr [%]   high-fat diet, ethanol   gene: Mthfr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 119 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mtr [%]   high-fat diet, ethanol   gene: Mtr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 98.5 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mttp [%]   high-fat diet, ethanol   gene: Mttp         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 141 (N=3)           • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Srebf1 [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 154 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred   liver — lipid homeostasis         lipid profile   Paigen1 hepatic cholesterol concentration [mg]   high-fat diet   8wks       • free  ...... 7.95 ▲▲   7.92 ▲         • esterified  ...... 61.7 ▲   59.3 ▲▲         • total  ...... 71.6 ▲▲   67.3 ▲▲         • free to esterified ratio  ...... 0.130   0.133         43 strains   age 15-19wks   Inbred Rusyn5 hepatic triglycerides (TG) [mg/g]   high-fat diet, ethanol       • high-fat only  ...... 4.79 (N=3)         • high-fat and ethanol  ...... 17.4 (N=3) ▼           • comparison   6 strains   age 8-14wks   Inbred   liver — pathology         histopathology   Paigen1 liver pathology (0-4) [score]   high-fat diet   8wks       • images  ......       • microvacuoles  ...... 3.00 (N=3)   1.67 (N=3)         • macrovacuoles  ...... 0 (N=3)   0 (N=3)         • inflammation  ...... 0.333 (N=3)   0.667 (N=3)         43 strains   age 15-19wks   Inbred Rusyn5 liver steatosis (0-4) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 0.833 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver inflammation (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 1.00 (N=3)         • high-fat and ethanol  ...... 1.00 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver necrosis (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 0 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 total liver histopathology (0-8) [score]   high-fat diet, ethanol       • high-fat only  ...... 1.00 (N=3)         • high-fat and ethanol  ...... 1.83 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         HPLC   Rusyn5 liver homocysteine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.177 (N=3) ▼         • high-fat and ethanol  ...... 0.240 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylhomocysteine (SAH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.223 (N=3)         • high-fat and ethanol  ...... 0.287 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver methionine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.720 (N=3)         • high-fat and ethanol  ...... 0.487 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylmethionine (SAM) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.600 (N=3)         • high-fat and ethanol  ...... 0.547 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of liver SAM to SAH (SAM/SAH) [ratio]   high-fat diet, ethanol       • high-fat only  ...... 2.68 (N=3)         • high-fat and ethanol  ...... 1.90 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         Western blot   Rusyn5 BHMT [%]   high-fat diet, ethanol   gene: Bhmt         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 89.4 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 DDIT3 (CHOP) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 105 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 HSPA5 (GRP78) [%]   high-fat diet, ethanol   gene: Hspa5         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 72.3 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 SREBF1 (NSREBP1) [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 115 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis — glutathione         HPLC   Rusyn5 reduced liver glutathione (GSH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 43.7 (N=3)         • high-fat and ethanol  ...... 36.2 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 oxidized liver glutathione (GSSG) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 2.18 (N=3)         • high-fat and ethanol  ...... 2.80 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of reduced to oxidized liver glutathione [ratio]   high-fat diet, ethanol       • high-fat only  ...... 21.9 (N=3)         • high-fat and ethanol  ...... 12.9 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — weight         scales   Paigen1 liver weight at sacrifice   [g] ...... 0.805 ▼   1.02 ▼     high-fat diet   8wks 43 strains   age 15-19wks   Inbred Rusyn5 liver weight as a percent of body weight [%]   high-fat diet, ethanol       • high-fat only  ...... 5.25 (N=3) ▼         • high-fat and ethanol  ...... 6.55 (N=3)           • comparison   14 strains   age 8-14wks   Inbred

128 measurements listed.