Home       About             Help       Phenotype data > LP/J strain > interventions > acetaminophen (pain reliever e.g. Tylenol)   See also:   Wikipedia Measurements: About these LP/J averages (♀ ♂) Exit LP/J mode blood — clinical chemistry — enzymes — ALT         metabolic panel   Threadgill1 alanine aminotransferase (serum ALT) [IU/L]     control and  acetaminophen       100 mg/kg:             • control  ...... 29.0 (N=2)               • 4h  ...... 36.3 (N=3) ▼               • 12h  ...... 96.3 (N=3) ▲               • 24h  ...... 93.0 (N=3) ▲▲                 • time comparison   37 strains   age 8-17wks   Inbred Threadgill2 alanine aminotransferase (serum ALT) [IU/L]   acetaminophen   50mg/kg       • 50 mg/kg  ...... 28.2 ▼         • 300 mg/kg  ...... 3101 (N=4)           • comparison   5 strains   age 6-8wks   Inbred   blood — clinical chemistry — enzymes — AST         metabolic panel   Threadgill1 aspartate aminotransferase (serum AST) [IU/L]     control and  acetaminophen       • control  ...... 108 (N=2)         post-Rx mg/kg:             • 100  ...... 331 (N=3) ▲▲               • 200  ...... 330 (N=3) ▼               • 300  ...... 166 (N=3) ▼                 • comparison   37 strains   age 8-17wks   Inbred   blood — clinical chemistry — urea nitrogen         metabolic panel   Threadgill1 blood urea nitrogen (BUN) [mg/dL]     control and  acetaminophen       • control  ...... 15.0 (N=1)         post-Rx mg/kg:             • 100  ...... 14.7 (N=3) ▼               • 200  ...... 21.3 (N=3)               • 300  ...... 17.7 (N=3)                 • comparison   36 strains   age 8-17wks   Inbred   blood — xenobiotics — drug and metabolite concentration         HPLC   Threadgill2 serum acetominophen (APAP) [µg/mL]     baseline and  acetaminophen 50mg/kg       • baseline  ...... 2.66         • 0.5h  ...... 5.48         • 1h  ...... 1.96         • 2h  ...... 0.562         • 3h  ...... 0.520         • 6h  ...... 0.366 ▲           • time comparison   5 strains   age 6-8wks   Inbred Threadgill2 serum acetaminophen sulfate (APAP-sulfate) [µg/mL]     baseline and  acetaminophen 50mg/kg       • baseline  ...... 0.646 ▼         • 0.5h  ...... 7.05 ▲         • 1h  ...... 5.60 ▲         • 2h  ...... 2.03         • 3h  ...... 1.22 ▼         • 6h  ...... 1.25 ▼           • time comparison   5 strains   age 6-8wks   Inbred Threadgill2 serum acetaminophen glucuronide (APAP-Glc) [µg/mL]     baseline and  acetaminophen 50mg/kg       • baseline  ...... 2.45         • 0.5h  ...... 31.0         • 1h  ...... 16.2         • 2h  ...... 4.95 ▼         • 3h  ...... 3.26         • 6h  ...... 3.38 (N=4)           • time comparison   5 strains   age 6-8wks   Inbred Threadgill2 serum acetominophen (APAP) [µg/mL]     baseline and  acetaminophen 300mg/kg       • baseline  ...... 38.0 (N=4)         • 0.5h  ...... 128 (N=4) ▲         • 1h  ...... 99.1 (N=4) ▲         • 2h  ...... 46.3 (N=4) ▲         • 3h  ...... 28.8 (N=4) ▲         • 6h  ...... 13.6 (N=4) ▲           • time comparison   5 strains   age 6-8wks   Inbred Threadgill2 serum acetaminophen sulfate (APAP-sulfate) [µg/mL]     baseline and  acetaminophen 300mg/kg       • baseline  ...... 2.20 (N=4)         • 0.5h  ...... 17.8 (N=4)         • 1h  ...... 25.1 (N=4)         • 2h  ...... 31.1 (N=4) ▲         • 3h  ...... 31.1 (N=4) ▲         • 6h  ...... 18.2 (N=3) ▲           • time comparison   5 strains   age 6-8wks   Inbred Threadgill2 serum acetaminophen glucuronide (APAP-Glc) [µg/mL]     baseline and  acetaminophen 300mg/kg       • baseline  ...... 11.3 (N=4)         • 0.5h  ...... 220 (N=4)         • 1h  ...... 344 (N=4)         • 2h  ...... 270 (N=4) ▲         • 3h  ...... 206 (N=4) ▲         • 6h  ...... 63.8 (N=3) ▲           • time comparison   5 strains   age 6-8wks   Inbred   liver — enzyme homeostasis         ELISA   Threadgill1 cytochrome P450 1A2 isoform [pmol/mg]     control and  acetaminophen 300 mg/kg       • control  ...... 104 (N=3) ▼         • post-Rx 300 mg/kg  ...... 145 (N=3)           • comparison   36 strains   age 8-12wks   Inbred Threadgill1 cytochrome P450 2E1 isoform [pmol/mg]     control and  acetaminophen 300 mg/kg       • control  ...... 88.2 (N=3)         • post-Rx 300 mg/kg  ...... 89.7 (N=3)           • comparison   36 strains   age 8-12wks   Inbred   liver — enzyme homeostasis         HPLC   Threadgill1 glutathione transferase [µg/mL]     control and  acetaminophen 300 mg/kg       • control  ...... 537 (N=3)         • post-Rx 300 mg/kg  ...... 762 (N=3)         36 strains   age 8-12wks   Inbred   liver — enzyme homeostasis         metabolic panel   Threadgill1 catalase [µg/mL]     control and  acetaminophen 300 mg/kg       • control  ...... 457 (N=3)         • post-Rx 300 mg/kg  ...... 393 (N=3)           • comparison   36 strains   age 8-12wks   Inbred   liver — pathology         histopathology   Threadgill1 necrosis [%]     control and  acetaminophen       • control  ...... 0 (N=3)         post-Rx mg/kg:             • 100  ......             • 200  ......             • 300  ...... 10.7 (N=3)                 • comparison   37 strains   age 8-17wks   Inbred Threadgill2 liver necrosis score   [%] ...... 45.9 (N=4)     acetaminophen   300mg/kg 5 strains   age 6-8wks   Inbred Threadgill2 percent nitrotyrosine staining (marker of hepatocyte injury)   [%] ...... 32.6 (N=4) ▼     acetaminophen   300mg/kg 5 strains   age 6-8wks   Inbred   liver — protein homeostasis — glutathione         HPLC   Threadgill1 reduced glutathione (GSH) [µmol/g]     control and  acetaminophen       • control  ...... 5.00 (N=2)         • post-Rx 300mg/kg  ...... 1.31 (N=4)           • comparison   35 strains   age 8-17wks   Inbred Threadgill1 oxidized glutathione (GSSG) [µmol/g]     control and  acetaminophen       • control  ...... 0.253 (N=2)         • post-Rx 300mg/kg  ...... 0.160 (N=4)           • comparison   35 strains   age 8-17wks   Inbred Threadgill1 total glutathione (GSH plus GSSG) [µmol/g]     control and  acetaminophen       • control  ...... 5.50 (N=2)         • post-Rx 300 mg/kg  ...... 1.63 (N=4)           • comparison   37 strains   age 8-17wks   Inbred Threadgill1 ratio of reduced (GSH) to oxidized (GSSG) glutathione [ratio]     control and  acetaminophen       • control  ...... 27.2 (N=2)         • post-Rx 300mg/kg  ...... 8.09 (N=4)           • comparison   35 strains   age 8-17wks   Inbred Threadgill2 reduced glutathione (GSH) [µmol/mg]     control and  acetaminophen 300mg/kg       • control  ...... 10.1 (N=3)         • post-Rx 300 mg/kg  ...... 5.08           • comparison   5 strains   age 6-8wks   Inbred Threadgill2 percent reduced glutathione [%]     control and  acetaminophen 300mg/kg       • control  ...... 81.2 (N=3) ▼         • post-Rx 300 mg/kg  ...... 71.6 ▼           • comparison   5 strains   age 6-8wks   Inbred Threadgill2 total glutathione [µmol/mg]     control and  acetaminophen 300mg/kg       • control  ...... 13.5 (N=3) ▲         • post-Rx 300 mg/kg  ...... 7.22           • comparison   5 strains   age 6-8wks   Inbred   liver — weight         scales   Threadgill1 liver weight [g]     control and  acetaminophen       • control  ...... 0.922         24h post-Rx mg/kg:             • 100  ...... 1.23 (N=3)               • 200  ...... 0.867 (N=3) ▼               • 300  ...... 1.00 (N=3)                 • comparison   37 strains   age 8-17wks   Inbred

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