liver — enzyme homeostasis         ELISA   Threadgill1 cytochrome P450 1A2 isoform [pmol/mg]     control and  acetaminophen 300 mg/kg       • control  ...... 197 (N=2)         • post-Rx 300 mg/kg  ...... 171 (N=3)           • comparison   36 strains   age 8-12wks   Inbred Threadgill1 cytochrome P450 2E1 isoform [pmol/mg]     control and  acetaminophen 300 mg/kg       • control  ...... 103 (N=2)         • post-Rx 300 mg/kg  ...... 64.1 (N=3)           • comparison   36 strains   age 8-12wks   Inbred   liver — enzyme homeostasis         HPLC   Threadgill1 glutathione transferase [µg/mL]     control and  acetaminophen 300 mg/kg       • control  ...... 693 (N=2)         • post-Rx 300 mg/kg  ...... 645 (N=4)         36 strains   age 8-12wks   Inbred   liver — enzyme homeostasis         metabolic panel   Threadgill1 catalase [µg/mL]     control and  acetaminophen 300 mg/kg       • control  ...... 627 (N=2)         • post-Rx 300 mg/kg  ...... 582 (N=4) ▲           • comparison   36 strains   age 8-12wks   Inbred   liver — gene expression         RT-PCR   Rusyn5 Acox1 [%]   high-fat diet, ethanol   gene: Acox1         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Adipor2 [%]   high-fat diet, ethanol   gene: Adipor2         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Ahcy [%]   high-fat diet, ethanol   gene: Ahcy         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 89.5 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cbs [%]   high-fat diet, ethanol   gene: Cbs         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 51.4 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cebpa [%]   high-fat diet, ethanol   gene: Cebpa         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 53.6 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Cth [%]   high-fat diet, ethanol   gene: Cth         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 106 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Ddit3 (Chop) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 57.4 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Gnmt [%]   high-fat diet, ethanol   gene: Gnmt         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 89.6 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mat1a [%]   high-fat diet, ethanol   gene: Mat1a         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 123 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mthfr [%]   high-fat diet, ethanol   gene: Mthfr         • high-fat only  ...... 100.0 (N=3)         • high-fat and ethanol  ...... 24.3 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mtr [%]   high-fat diet, ethanol   gene: Mtr         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 54.6 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 Mttp [%]   high-fat diet, ethanol   gene: Mttp         • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Rusyn5 Srebf1 [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 53.6 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — lipid homeostasis         lipid profile   Rusyn5 hepatic triglycerides (TG) [mg/g]   high-fat diet, ethanol       • high-fat only  ......       • high-fat and ethanol  ......         • comparison   6 strains   age 8-14wks   Inbred Schonfeld1 hepatic triglycerides (5h fast)   [mg/g] ...... 283 ▲▲   384 ▲▲   10 strains   age 9-13wks   Inbred   liver — mineral homeostasis         mass spectrometry   Vulpe1 liver mineral [µg/g]         • copper  ...... 12.5   16.8         • iron  ...... 439   317         • zinc  ...... 73.6   70.0           • comparison   18 strains   age 8wks   Inbred   liver — pathology         histopathology   Rusyn5 liver steatosis (0-4) [score]   high-fat diet, ethanol       • high-fat only  ...... 0.167 (N=3)         • high-fat and ethanol  ...... 2.33 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver inflammation (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 0.667 (N=3)         • high-fat and ethanol  ...... 0.667 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver necrosis (0-2) [score]   high-fat diet, ethanol       • high-fat only  ...... 0 (N=3)         • high-fat and ethanol  ...... 0.667 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 total liver histopathology (0-8) [score]   high-fat diet, ethanol       • high-fat only  ...... 0.833 (N=3)         • high-fat and ethanol  ...... 3.67 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Threadgill1 necrosis [%]     control and  acetaminophen       • control  ...... 0 (N=2)         post-Rx mg/kg:             • 100  ......             • 200  ......             • 300  ...... 32.5 (N=4)                 • comparison   37 strains   age 8-17wks   Inbred   liver — protein homeostasis         HPLC   Rusyn5 liver homocysteine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.247 (N=3) ▲         • high-fat and ethanol  ...... 0.327 (N=3) ▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylhomocysteine (SAH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.303 (N=3) ▲         • high-fat and ethanol  ...... 0.350 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver methionine [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.643 (N=3) ▼         • high-fat and ethanol  ...... 0.417 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 liver S-adenosylmethionine (SAM) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 0.553 (N=3) ▼         • high-fat and ethanol  ...... 0.470 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of liver SAM to SAH (SAM/SAH) [ratio]   high-fat diet, ethanol       • high-fat only  ...... 1.84 (N=3) ▼         • high-fat and ethanol  ...... 1.36 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis         Western blot   Rusyn5 BHMT [%]   high-fat diet, ethanol   gene: Bhmt         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 81.3 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 DDIT3 (CHOP) [%]   high-fat diet, ethanol   gene: Ddit3         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 102 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 HSPA5 (GRP78) [%]   high-fat diet, ethanol   gene: Hspa5         • high-fat only  ...... 100 (N=2)         • high-fat and ethanol  ...... 126 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 SREBF1 (NSREBP1) [%]   high-fat diet, ethanol   gene: Srebf1         • high-fat only  ...... 100 (N=3)         • high-fat and ethanol  ...... 92.0 (N=3)           • comparison   14 strains   age 8-14wks   Inbred   liver — protein homeostasis — glutathione         HPLC   Rusyn5 reduced liver glutathione (GSH) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 48.3 (N=3)         • high-fat and ethanol  ...... 26.7 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 oxidized liver glutathione (GSSG) [nmol/mg]   high-fat diet, ethanol       • high-fat only  ...... 2.34 (N=3) ▲         • high-fat and ethanol  ...... 2.97 (N=3) ▲▲           • comparison   14 strains   age 8-14wks   Inbred Rusyn5 ratio of reduced to oxidized liver glutathione [ratio]   high-fat diet, ethanol       • high-fat only  ...... 20.8 (N=3) ▼         • high-fat and ethanol  ...... 9.43 (N=3) ▼           • comparison   14 strains   age 8-14wks   Inbred Threadgill1 reduced glutathione (GSH) [µmol/g]     control and  acetaminophen       • control  ...... 2.75 (N=2) ▼         • post-Rx 300mg/kg  ...... 0.553 (N=4) ▼           • comparison   35 strains   age 8-17wks   Inbred Threadgill1 oxidized glutathione (GSSG) [µmol/g]     control and  acetaminophen       • control  ...... 0.179 (N=2)         • post-Rx 300mg/kg  ...... 0.0490 (N=4) ▼           • comparison   35 strains   age 8-17wks   Inbred Threadgill1 total glutathione (GSH plus GSSG) [µmol/g]     control and  acetaminophen       • control  ...... 3.11 (N=2) ▼         • post-Rx 300 mg/kg  ...... 0.651 (N=4) ▼           • comparison   37 strains   age 8-17wks   Inbred Threadgill1 ratio of reduced (GSH) to oxidized (GSSG) glutathione [ratio]     control and  acetaminophen       • control  ...... 15.3 (N=2)         • post-Rx 300mg/kg  ...... 67.3 (N=4) ▲▲           • comparison   35 strains   age 8-17wks   Inbred   liver — weight         scales   Harrill2 liver weight at sacrifice [g]     control and  DB289       • control  ...... 0.728         • DB289  ...... 0.584           • comparison   34 strains   age 6-8wks   Inbred Harrill2 liver weight as percent of body weight [%]     control and  DB289       • control  ...... 4.08         • DB289  ...... 3.62         • difference  ...... 0.887         34 strains   age 6-8wks   Inbred Jaxpheno2 liver weight [g]         • age 8wks  ...... 1.14 ▲   1.37         • age 16wks  ...... 1.41 ▲   1.41           • age comparison   11 strains   ages shown   Inbred Jaxpheno2 liver weight as percent of body weight [%]         • age 8wks  ...... 5.88 ▲   5.72 ▲         • age 16wks  ...... 5.74 ▲   4.87           • age comparison   11 strains   ages shown   Inbred Rusyn5 liver weight as a percent of body weight [%]   high-fat diet, ethanol       • high-fat only  ...... 6.45 (N=3)         • high-fat and ethanol  ...... 7.91 (N=3)           • comparison   14 strains   age 8-14wks   Inbred Schonfeld1 liver weight   [g] ...... 1.08   1.49 ▲▲   10 strains   age 9-13wks   Inbred Schonfeld1 liver weight as a percent of body weight   [%] ...... 5.21 ▲   5.27 ▲   10 strains   age 9-13wks   Inbred Threadgill1 liver weight [g]     control and  acetaminophen       • control  ...... 1.61 (N=4) ▲         24h post-Rx mg/kg:             • 100  ......             • 200  ......             • 300  ...... 1.77 (N=4) ▲                 • comparison   37 strains   age 8-17wks   Inbred

101 measurements listed.